I have to use this software, Quantity One (Bio-Rad), to quantify my western results... After going through all the steps and click on "report", I am very confuse with the numbers that I got--
I use local background substraction and all the bands are selected using boxes with equal size.It seems to me that "Adj. Vol. INT*mm2" is what I need for plotting, but I don't understand why the numbers are in negative values.
Any input from those who are familiar with this software are truly appreciated!