cell lysis

Hi all,

Could anybody tell and guide me to the best protocol for cell lysis (At the end my needs are nuclear proteins). I have quite a number of protocols with me but confused which one would be best for protein extraction. I am working on adherent cells and need a protocol that will serve my purpose.

Thanks,

V

Vdas please read this article: Andrews, N.C. and Faller, D.V. (1991) A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. Nucleic Acids Research 19, 2499

Even after that you don't find a suitable solution, please contact me again. I will try my best.

Dear Vdas,

Cell lysis protocol for proteins.

Visit this website...This may fulfil your needs.

http://labs.pbrc.edu/endocrinology/documents/CELLLYSISPROTOCOLFORPROTEIN2.pdf

Hi all,

is the protocol described for extraction of DNA-binding proteins good for Mass spec studies  also ??

I am interersted in whole protein extraction instead of organelle proteins. I am trying various methods of protein extraction.

Please suggest which lysis buffer and method one would  be the best ??

Thanks

Preety

 The article of Andrews & Faller of 1991 clearly answers the question. For mass spec studies I am not sure because both have different protocols.

This is a good kit for nuclear protein extraction

http://www.piercenet.com/products/browse.cfm?fldID=06010435