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help me to prepare urine protein sample preparation in 2D gel Electrophoresis

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nalpy
India

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Topic Started by nalpy
on 6/2/2009 19:56 PM   
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Can any one send the details protocol to prepare urine protein sample preparation in 2D gel Electrophrosis and already not not work in crude protein sample.


Last edited Jun 03, 2009, 9:44 AM by R Bishop
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pundir
India

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Posted By pundir
on 6/3/2009 11:22 AM   
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Hi nalpy,

you can find some relevent information in the articles mentioned below

"Systematic Evaluation of Sample Preparation Methods for Gel-Based Human Urinary Proteomics: Quantity, Quality, and Variability" pubs.acs.org/doi/pdf/10.1021/pr0502525

 

"Proteomic analysis of normal human urinary proteins isolated by acetone precipitation or ultracentrifugation"  www.nature.com/ki/journal/v62/n4/pdf/4493245a.pdf

impossible! let's do it................



ARGERINE
India

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Posted By ARGERINE
on 6/3/2009 2:26 AM   
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Hi Nalpy

There are alot of published research articles and reviews in the field of Urinary proteome analysis. Especially During the HUPOs plasma proteome project in Proteomics and Journal of proteomics as well. I have alot of those articles with me as Im working in the field of plasma proteomics.

Few of the key links are

http://jasn.asnjournals.org/cgi/reprint/18/4/1057

http://www.mcponline.org/cgi/content/full/5/10/1760

http://ajprenal.physiology.org/cgi/reprint/287/3/F353.pdf

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2215359

http://www3.interscience.wiley.com/journal/107629553/abstract?CRETRY=1&SRETRY=0

In case you dont have access to these journals send me your email ID I will enclose the articles to you.

All the best

Gaganjot Singh Truth seems so closer now......



nalpy
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Posted By nalpy
on 6/3/2009 7:31 AM   
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Dear Pundir & Argerine

Thank you for your kind suggestion and papers link


Last edited Jun 03, 2009, 10:40 AM by varsha

varsha
United States


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Posted By varsha
on 6/3/2009 14:01 PM   
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Hi Nalpy.

I knew a student who did it. It involves acetone pptn of the urine sample. Then you can up the ppt in PBS  for protein estimation or directly into 2D lysis buffer. The protocol is described in the following paper if you can get it from the library . Good luck!

Varsha



nalpy
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Posted By nalpy
on 6/3/2009 20:02 PM   
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Dear  Dr. Varsha

Thank you for your kind help and follow the protocol.

Regards

palanisamy



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