Genome-Wide siRNA screening [View Printable]
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roudi
Group: Member
Posts: 69
Joined: Dec 14, 2004
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siRNA research and tools are blooming. Many companies offer state of the Art technologies regarding RNAi for diagnostics and therapeutic products. Novartis Institute for Biomedical Research(www.novartis.com) screens siRNA in a large scale to identify genes involved in cell-signaling pathways and also to identify new genes involved in regulatory pathways. The method used is identifying gene functions via high throughput cell-based assays.
Please anyone knows about other companies with products regarding RNAi technologies? |
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| Posted Jun 20, 2005, 20:27 PM |
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David E
Group: Member
Posts: 263
Joined: Sep 29, 2004
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Dharmacon, Qiagen, Ambion, Invitrogen
are the largest providers of RNAi technology products
others include
MWG, IDT and Open Biosystems |
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| Posted Aug 11, 2005, 14:26 PM |
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DD
Group: Member
Posts: 64
Joined: Dec 17, 2004
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Has anyone used the TriFECTa Dicer-Substrate RNAi kit from Integrated DNA Technologies?. They claim to provide a 70% knockdown of the target mRNA when >90% transfection efficiency.
The kit has three target-specific Dicer-Substrate siRNA duplexes (5 nmoles each), Cy3TM DS Transfection Control, fluorescent-labeled transfection control duplex (1nmole), HPRT-S1 DS positive Control Duplex (1nmole), DS Scrambled-Neg, universal negative control duplex (1nmole). RNase-free Duplex buffer. |
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| Posted Dec 12, 2005, 22:44 PM |
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parvoman
Group: Member
Posts: 272
Joined: Jul 28, 2005
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| David E said: | Dharmacon, Qiagen, Ambion, Invitrogen
are the largest providers of RNAi technology products
others include
MWG, IDT and Open Biosystems |
...better add Roche's Xtreme and Epoch Biolabs' GeneCarrier and RNACarrier reagents to that list. The latter has been especially good in my hands with endothelial cells (which are difficult). |
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| Posted Dec 13, 2005, 19:46 PM |
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