related to translation

Two populations of RNAs are made by the random combination of nucleotides .In population A ,the RNAs contain only A & G nucleotides (3 A: 1G),where as in population B ,the RNAs contain only A & U nucleotides(3A: 1U).In what ways the proteins produced by translating the population A RNAs differs from those produced by translating population B RNAs.

Hi.

This sounds like a question from an exam paper. You should attempt to do this in following manner:

1. For population A, find all the codons that contain just Aa, just Gs or a combination of A and G and the amino acids they code for

2. make a mix of those codons so you satisfy 3A:1G ratio

3. Find the mix of amino acids this RNA would code for

4. Repeat steps 1-3 for population B.

5. Compare the aminoacid composition of the two population and you should be able to talk about the pI of the two proteins  and their hydrophobic/hydrophilic nature etc.

Let us know what you come up with.

good afternoon sir,
actually its a design problem to me i want to simply know,wheather there is any effect on the protein produced by the translation of the two types of rna which are differ only in their nucleotide sequence

I guess it would depend upon the translation mix and the concentration of acyl tRNAs therein.

For example in yeast ( I am quoting Voet) only 25 of the 61  coding triplets are commonly used. That meands that these 25 tRNAs are more aboundant in the cytosol than the rest. So you would like to use these codons in your sequence for optimal translation.

If you are using rabbit reticulocyte lysate or E. coli for in vitro translation/recombinant protein expression, you should optimize/design the codons so that you have more of favored codons.

You frequently have to codon optimization/swap when you want to express protein from an AT rich (>60%) genome e.g Plasmodium falciparum into E. coli or other relatively GC rich systems.

In toyr case, you need to decide based on where you want to express the protein.

Hope this is helpful.