http://www.scientistsolutions.com/t16973-virus+concentration_purification.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t16973-virus+concentration_purification.html Purification of virus means removing all the residual junk that is in your virus preparation. eg. cell debris, protein molecules. Purifying can be done by chromatography, centrifugation and dialysis.
Concentration of virus means increasing the virus titre you have in the solution. Usually this means some kind of reduction in volume. Methods I have used are ultracentrifugation in a sucrose gradient, PEG precipitation, and Vivaflow ultrafiltration.
I hope that help...]]> Tue, 07 Dec 2010 16:47:47 GMT http://www.scientistsolutions.com/t16973-virus+concentration_purification.html Tue, 07 Dec 2010 01:15:27 GMT http://www.scientistsolutions.com/t16973-virus+concentration_purification.html What different methods can be used to obtain a concentrated virus suspension and a purified virus concentration?
Centrifugation, filtration, adsorption, column chromatography, affinity chromatography, dialysis?
Cheers! :)]]> Mon, 06 Dec 2010 13:07:24 GMT