Partial adherent cell fixation http://www.scientistsolutions.com/t1183-partial+adherent+cell+fixation.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 Partial adherent cell fixation http://www.scientistsolutions.com/t1183-partial+adherent+cell+fixation.html We did try methanol/acetone 1:1 at one point & that seemed to work fine as well (we were trying to troubleshoot a particular antibody that was giving us a headache).
I don't know if the addition of acetone would assist your situation, but might be worth a try if the PFA doesn't work.]]> Mon, 10 Oct 2005 06:33:03 GMT Partial adherent cell fixation http://www.scientistsolutions.com/t1183-partial+adherent+cell+fixation.html Agreed.... 4% PFA has always worked for me too... you might need to rinse off the PFA/PBS with whatever buffer base you are using for the stain IF there is some reaction or irregular staining with the PBS by the staining reagent.
The cells shrink due to the methanol being too 'drying'......]]> Tue, 30 Aug 2005 03:38:10 GMT Partial adherent cell fixation http://www.scientistsolutions.com/t1183-partial+adherent+cell+fixation.html Wed, 10 Aug 2005 15:22:37 GMT Partial adherent cell fixation http://www.scientistsolutions.com/t1183-partial+adherent+cell+fixation.html Fix with paraformaldehyde may be help, but if it compatible to staining solution? As I need to fix the cell and then stain the nucleus and cytoplasm. THX!]]> Wed, 10 Aug 2005 01:43:31 GMT Partial adherent cell fixation http://www.scientistsolutions.com/t1183-partial+adherent+cell+fixation.html Inclusion of small amounts of glutaraldehyde (0.1%) along with paraformaldehyde in the fixation step usually helps in preservation of cell structure and surface membrane proteins following permeabilisation by Triton X-100. In this case it may be necessary to determine if antigenicity is also maintained after glutaraldehyde is added.
If Tri...]]> Tue, 09 Aug 2005 15:23:45 GMT Partial adherent cell fixation http://www.scientistsolutions.com/t1183-partial+adherent+cell+fixation.html I am now doing with a cell line PU5-1.8, a marcophage like cell line. I want to fix it on cell culture treated coverslip and take to observe under microscope. But when I add mentanol to fix it, the cells turn to shrink and look ugly, just like a debris. Do anyone can give me some advice?
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