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<title>Immunology Protocol</title>
<link>http://www.scientistsolutions.com/a25-protocolslist-immunology.aspx</link>
<description>Discussion of the Immune System including T cells, B cells, macrophages, Natural Killer T cells, bone marrow, chemokines, cytokines, innate immunity, blood types, immune response, inflammation…
 Life Science Discussion</description>
<language>en-us</language> 
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<sy:updateBase>2005-05-05T12:00+00:00</sy:updateBase>
<item>
<title>Allergies</title>
<link>http://www.scientistsolutions.com/a7388-protocol-allergies.aspx</link>
<description><![CDATA[Can a person become allergic to their own blood through a tatoo?]]></description>
<pubDate>Tue, 23 Oct 2012 13:06:09 GMT</pubDate>
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<title>Maintaining Lymphoblastoid Cell Lines</title>
<link>http://www.scientistsolutions.com/a7261-protocol-maintaining+lymphoblastoid+cell+lines.aspx</link>
<description><![CDATA[This protocol is explains about growth of  lymphoblastoid cells for permanent storage and for DNA extraction.<br />Safety Considerations:<br />  All cultured animal and human cells have the potential for carrying viruses, latent viral genomes, and other infectious agents. Cell cultures should be handled very carefully by trained persons under laboratory conditions which afford adequate biohazard containment. A Biological Safety Cabinet must be used when ...]]></description>
<pubDate>Wed, 25 Nov 2009 06:44:41 GMT</pubDate>
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<title>The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro</title>
<link>http://www.scientistsolutions.com/a7211-protocol-the+op9_dl1+system_+generation+of+t_lymphocytes+from+embryonic+or+hematopoietic+stem+cells+in+vitro.aspx</link>
<description><![CDATA[Differentiation of mouse embryonic stem cells (ESCs) or hematopoietic stem cells (HSCs) from fetal liver or bone marrow into T-lymphocytes can be achieved in vitro with the support of OP9-DL1 cells, a bone-marrow-derived stromal cell line that ectopically expresses the Notch ligand, Delta-like 1 (Dll1). This approach provides a simple, versatile, and efficient culture system that allows for the commitment, differentiation, and proliferation of T-lineage cells from different sources of stem cells...]]></description>
<pubDate>Tue, 29 Sep 2009 15:41:01 GMT</pubDate>
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<title>Elution of HBsAg from aluminum hydroxide adjuvant</title>
<link>http://www.scientistsolutions.com/a7160-protocol-elution+of+hbsag+from+aluminum+hydroxide+adjuvant.aspx</link>
<description><![CDATA[The elution of HBsAg from aluminum hydroxide adjuvant is studied using sheep lymph fluid. <br />Lymph fluid is identical to interstitial fluid [1] and is harvested using a previously described method [2]. <br />A model vaccine containing 1.62 mg Al/ml and 1 &amp;mu;g HBsAg/ml is prepared at pH 7.4. <br />The vaccine (0.6 ml) is mixed with 2.4 ml of sheep lymph fluid at 37 &amp;deg;C using a shaking water bath at 37 &amp;deg;C. <br />Samples are withdrawn at 0, 1, 6...]]></description>
<pubDate>Thu, 03 Sep 2009 09:21:39 GMT</pubDate>
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<title>Experimental Models of Hypoxic-Ischemic Encephalopathy, Hypoxia-Ischemia in the Immature Rat</title>
<link>http://www.scientistsolutions.com/a5923-protocol-experimental+models+of+hypoxic_ischemic+encephalopathy%2c+hypoxia_ischemia+in+the+immature+rat.aspx</link>
<description><![CDATA[The development of experimental models to study the mechanisms of perinatal hypoxic-ischemic encephalopathy and stroke and effective therapies represents an important goal in perinatal medicine. However, due to the complexity of this pathological condition in humans, to date there is no ideal animal model that completely reproduces this condition. This unit describes the most widely used rodent animal model for the study of hypoxic-ischemic encephalopathy during development. The model consists o...]]></description>
<pubDate>Mon, 06 Jul 2009 16:34:19 GMT</pubDate>
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<title>Human Complement Components C4A and C4B Genetic Diversities, Complex Genotypes and Phenotypes</title>
<link>http://www.scientistsolutions.com/a5929-protocol-human+complement+components+c4a+and+c4b+genetic+diversities%2c+complex+genotypes+and+phenotypes.aspx</link>
<description><![CDATA[This unit describes methods that can accurately determine the genotypes and phenotypes of human complement components C4A and C4B. Specifically, they allow investigators to determine how many C4 genes are present in a diploid genome of a human subject and to quantify how many of them encode C4A proteins and how many of them encode C4B proteins. In addition, methods to determine how many long and short C4 genes are present in a diploid genome of a subject are described together with experimental ...]]></description>
<pubDate>Mon, 06 Jul 2009 16:34:19 GMT</pubDate>
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<title>Measurement of Interleukin-21</title>
<link>http://www.scientistsolutions.com/a5881-protocol-measurement+of+interleukin_21.aspx</link>
<description><![CDATA["This unit describes three procedures for measurement of interleukin-21 (IL-21). The first employs the use of an antibody sandwich ELISA. An alternative procedure measures proliferative responses of T cells to a combination of IL-21 and IL-15 using CFSE. Finally, a method to assess IL-21-induced tyrosine phosphorylation of Stat3 in splenic CD8+ T cells using a flow cytometry ""based analysis is described. Curr. Protoc. Immunol. 78]]></description>
<pubDate>Mon, 06 Jul 2009 16:31:11 GMT</pubDate>
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<item>
<title>Measurement of Peptide Dissociation from MHC Class II Molecules</title>
<link>http://www.scientistsolutions.com/a5885-protocol-measurement+of+peptide+dissociation+from+mhc+class+ii+molecules.aspx</link>
<description><![CDATA["This unit describes a method of measuring the kinetic stability of complexes formed between purified MHC class II molecules and antigenic N-terminal fluorescein-labeled peptides. An HPLC-SEC allows for the separation of fluoresceinated MHC class II]]></description>
<pubDate>Mon, 06 Jul 2009 16:31:11 GMT</pubDate>
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<item>
<title>Flow Rate Calibration for Absolute Cell Counting Rationale and Design</title>
<link>http://www.scientistsolutions.com/a5871-protocol-flow+rate+calibration+for+absolute+cell+counting+rationale+and+design.aspx</link>
<description><![CDATA[There is a need for absolute leukocyte enumeration in the clinical setting, and accurate, reliable (and affordable) technology to determine absolute leukocyte counts has been developed. Such technology includes single platform and dual platform approaches. Derivations of these counts commonly incorporate the addition of a known number of latex microsphere beads to a blood sample, although it has been suggested that the addition of beads to a sample may only be required to act as an internal qual...]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:20 GMT</pubDate>
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<title>Purification of T Cell Subpopulations</title>
<link>http://www.scientistsolutions.com/a5848-protocol-purification+of+t+cell+subpopulations.aspx</link>
<description><![CDATA[This unit describes a procedure for isolating T cell populations or subpopulations using the method of indirect panning. In this method, cells are selected by their capacity to bind to antibody-coated plates on the basis of particular cell-surface markers. It is superior to the antibody/complement lysis method (also presented) because the nonselected cell population can be retrieved.]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
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<title>The Day-3 Thymectomy Model for Induction of Multiple Organ-Specific Autoimmune Diseases</title>
<link>http://www.scientistsolutions.com/a5861-protocol-the+day_3+thymectomy+model+for+induction+of+multiple+organ_specific+autoimmune+diseases.aspx</link>
<description><![CDATA[Autoimmune diseases of the ovary and the stomach are major components of the murine autoimmune polyendocrinopathy syndrome that occurs following experimental perturbation of the normal immune system. Several methods of perturbation can lead to autoimmune disease, and the disease occurs in multiple organs in association with autoantibodies to tissue-specific antigen of the respective organs. The autoimmune diseases develop in a variety of laboratory mouse strains that are not prone to spontaneous...]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
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<item>
<title>Proliferative Assays for B Cell Function</title>
<link>http://www.scientistsolutions.com/a5845-protocol-proliferative+assays+for+b+cell+function.aspx</link>
<description><![CDATA[This unit describes procedures for measuring the capacity of purified B cells to undergo proliferation. The method centers on the use of polyclonal stimulating agents (mitogens) because these agents stimulate the majority of B cells and because the alternative (measurement of antigen-induced proliferation) requires the laborious procedures of isolating antigen-specific B cells (which are otherwise present in too low a concentration in whole B cell populations). Cross-linking of the B cell antige...]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
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<item>
<title>Optical Microscopy ""Based Migration Assay for Human Neutrophils</title>
<link>http://www.scientistsolutions.com/a5836-protocol-optical+microscopy+__based+migration+assay+for+human+neutrophils.aspx</link>
<description><![CDATA[This unit describes an in vitro microscopy assay for examining the migration of human neutrophils in two dimensions to identify the underlying cause of a migration defect and to evaluate a variety of migration parameters that cannot be studied using migration through a porous filter. Freshly isolated human neutrophils a placed in the chamber, stimulated, and images are collected at various time points. The data can be used to determine the effects of a pharmacological treatment on the migration ...]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
</item>
<item>
<title>Vaccination of Mice with Baculovirus-Infected Insect Cells Expressing Antigenic Proteins</title>
<link>http://www.scientistsolutions.com/a5867-protocol-vaccination+of+mice+with+baculovirus_infected+insect+cells+expressing+antigenic+proteins.aspx</link>
<description><![CDATA["Methods to induce antigen-specific immune responses in mice using insect cells infected with recombinant baculoviruses are described in this unit. Although this vaccine strategy has been used to generate both antibody and T cell responses]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
</item>
<item>
<title>Proliferative Assays for T Cell Function</title>
<link>http://www.scientistsolutions.com/a5846-protocol-proliferative+assays+for+t+cell+function.aspx</link>
<description><![CDATA["This addition to unit 3.12 will describe the assays needed to evaluate CD4 ?CD25 ?T cell non-responsiveness and function. Unlike the conventional T cells described in Basic Protocol 1]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
</item>
<item>
<title>The Reporter Antigen Popliteal Lymph Node Assay</title>
<link>http://www.scientistsolutions.com/a5864-protocol-the+reporter+antigen+popliteal+lymph+node+assay.aspx</link>
<description><![CDATA["Many chemicals]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
</item>
<item>
<title>T Cell Enrichment by Cytotoxic Elimination of B Cells and Accessory Cells</title>
<link>http://www.scientistsolutions.com/a5858-protocol-t+cell+enrichment+by+cytotoxic+elimination+of+b+cells+and+accessory+cells.aspx</link>
<description><![CDATA[T cells from mouse spleen and lymph node do not express the cell-surface glycoproteins encoded for by MHC class II genes, whereas most non-T cells in these organs do (i.e., B cells and accessory cells). This unique feature of T cells makes it possible to enrich for them using cytotoxic anti-class II monoclonal antibodies and activating complement. Because there are two isotypes of MHC class II genes (A and E genes, located adjacent to each other in the MHC region originally called the I region),...]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
</item>
<item>
<title>Flow Cytometry of the Side Population (SP)</title>
<link>http://www.scientistsolutions.com/a5869-protocol-flow+cytometry+of+the+side+population+sp.aspx</link>
<description><![CDATA[The side population (SP) has become an important hallmark for the definition of the stem-cell compartment, especially for the detection of stem cells and for their physical isolation by fluorescence-activated cell sorting (FACS). SP cells are CD34-? and were discovered using ultraviolet excitation based on the efflux of Hoechst 33342 (Ho342). Although the method works as originally described, the protocol is difficult for most investigators to perform: first, because the ability to discriminate ...]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
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<item>
<title>Production of Mouse T Cell Hybridomas</title>
<link>http://www.scientistsolutions.com/a5843-protocol-production+of+mouse+t+cell+hybridomas.aspx</link>
<description><![CDATA[T cell hybridomas can be obtained by fusing activated T cells with tumor cells. A heterogeneous population of hybridomas can be cloned by limiting dilution to obtain hybridomas that express specificity to one T cell receptor (TCR). This unit describes cell fusion and selection of T cell hybridomas. A protocol is provided for screening of T cell hybridomas for expression of the CD3-TCR complex by flow cytometry analysis. Those hybridomas expressing a CD3-TCR complex are then further tested for ex...]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
</item>
<item>
<title>Peptide Binding Motifs for MHC Class I and II Molecules</title>
<link>http://www.scientistsolutions.com/a5838-protocol-peptide+binding+motifs+for+mhc+class+i+and+ii+molecules.aspx</link>
<description><![CDATA[This overview discusses the use of peptide-bindnig motifs to predict interaction with a specific MHC class I or II allele, and gives examples for the use of MHC binding motifs to predict T-cell recognition.]]></description>
<pubDate>Mon, 06 Jul 2009 16:29:19 GMT</pubDate>
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