http://www.scientistsolutions.com/t2419-to+get+rid+of+differentiated+cells.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t2419-to+get+rid+of+differentiated+cells.html If you need ONLY stem cells, then you're going to have to use FACS sorting, 2 rounds of it is often used in literature to make absolutely sure your sample is pure.
If you're just trying to reduce the number of differentiated cells, passing is the first step.... beyond that, perhaps try seeding to many new clonogenic colonies--and select one that has few enough undifferentiated cells.
Cheers
Luke
]]> Mon, 08 Feb 2010 14:10:02 GMT http://www.scientistsolutions.com/t2419-to+get+rid+of+differentiated+cells.html IS this a standard line used in many labs, or a novel/less well caracterized line?]]> Sat, 01 Jul 2006 06:32:44 GMT http://www.scientistsolutions.com/t2419-to+get+rid+of+differentiated+cells.html Sat, 24 Jun 2006 10:13:56 GMT http://www.scientistsolutions.com/t2419-to+get+rid+of+differentiated+cells.html Fri, 23 Jun 2006 15:05:14 GMT http://www.scientistsolutions.com/t2419-to+get+rid+of+differentiated+cells.html I am growing stem cells. But after a while i have a mixed culture of both differentiated and undifferentiated population. could you kindly tell me as to how can i get rid of the differentiated cells. i need only undifferentiated stem cells for my work
thanx for help
deb]]> Fri, 23 Jun 2006 13:26:44 GMT