http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html but unfortunately I didnot have any results,
Do my cells died?
can you help me?]]> Tue, 21 Feb 2012 07:33:41 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Tue, 22 Nov 2011 09:58:46 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Tue, 20 Sep 2011 10:24:45 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html i need to know the molecular effects DMSO has on blood vessels, more specifically the mesenteric artery? i have read some journals about how it inhibits sodium and calcium channels and some say it increases cell permeability. but none of these talked about the vasculature? it was about cancer cells and brain cells etc?
any help would be great thanks!
ps im looking for detailed info if u can!! ...]]> Sat, 17 Apr 2010 17:58:58 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Thu, 25 Feb 2010 23:27:48 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Most of the chemokines we use (e.g. MIP2) are water soluble. For the few that are not, we dissolve in a minimal volume (e.g 100-200 ul of TC grade DMSO ...]]> Thu, 25 Feb 2010 21:34:35 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Thu, 25 Feb 2010 19:15:57 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html I thaw my cells (previously froze in 1 ml freezing medium containing 10% DMSO) directly into a 10cm culture dish filled with 9ml of DMSO free medium, and replace their medium into a fresh one the next day. They seem fine... Is there something I can`t see?]]> Tue, 08 Dec 2009 03:44:28 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Fri, 24 Jul 2009 09:53:25 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html ]]> Thu, 23 Jul 2009 15:22:03 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Thu, 18 Jun 2009 05:25:09 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html I think you may
1. dilute the extract with complete medium
2. dialysis with complete medium to get rid of the DMSO
3.concentrate the extract by spin column.
4. filter sterile the extract.
1% DMSO doesn't cause any toxicity to cells but we generally use 0.5% DMSO, final concentration.
To keep the final concentration to 0.5%, you can make 200x stock in 100% DMSO.
Or to keep the final concentration of 1% DMSO, you can use 100x stock in 100% DMSO.
Hope it helps.
Tirumal]]> Thu, 18 Dec 2008 08:08:40 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html I got the solution for my problem and wht u r saying is right that DMSO increases cell permiability...
Actually, I was adding 0.1% DMSO for all the wells in assay plate except my agonist CRC, might be due to which my agonist values were lower than rest all (Max). Nw I am adding DMSO in all wells including agonist CRC and I am getting good results..
again thank u very much for your productive suggetion.]]> Tue, 16 Dec 2008 04:21:00 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html The cause could be DMSO is harmful to cellls especially at higher temp.
It is commonly used at conce 5-10% for cell preservation. Cells are maintained on ice when in a medium with DMSO.
DMSO is said to increase cell permiabiltiy. That may be interfering as it depends on conc of DMSO also.
If you could elborate at which stage you are using it I may help you.
]]> Fri, 12 Dec 2008 00:58:56 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html if for cell freeze storage, DMSO works as cryoprotectant, 10% will be good, and keep in mind DMSO is toxic to cells
if you use it for microscope, it is used to permeabilize cells, 0.5% will be good]]> Thu, 11 Dec 2008 08:46:32 GMT http://www.scientistsolutions.com/t8064-dmso+in+cell+based+assays.html Pls let me know about the effect of DMSO on cells with different concentrations and also it's role in synergism of agonist action if any...
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