http://www.scientistsolutions.com/c431-microscopy+and+imaging.html Discussion on scanning and transmission electron microscopy (SEM), atomic force microscopy (AFM), confocal, lenses, microscope brands, in vivo imaging, fluorescence, 2D rendering, imaging software, FRET, CT, MRI.. Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t26731-molecular+networks+is+hiring_+electrophysiology%2c+ht+microscopy%2c+imaging.html We are currently hiring in our Molecular networks team.
If you specialize in the areas of Electrophysiology, HT Microscopy...]]> Tue, 30 Apr 2013 12:30:56 GMT http://www.scientistsolutions.com/t26537-searching+for+aluminium+planchettes.html I am trying to track down some Aluminium planchettes, 1 1/4", for use in histology/electron microscopy. Our previous supplier (Agar scientific) has discontinued them, along with any other suppliers I have been able to find, such as Quorum Technologies and Fisher Scientific. If anyone can please advise me as to where I can buy them from it would be much appreciated.
Many thanks,
Jo]]> Thu, 04 Apr 2013 08:08:45 GMT http://www.scientistsolutions.com/t26285-problem+with+gamma+h2ax+foci+staining.html I am a grad student & am having some problem in Gamma H2AX staining.

My interest is to see the effect of my gene on DNA damage after irradiation (5 Gy).
My problem is even my control cells (non-irradiated) showing many foci (some cells more than 10).
Here is my experimental setup
Cells: Mouse fibroblast cell line transfected with Human gene.
Un...]]> Wed, 20 Mar 2013 09:44:15 GMT http://www.scientistsolutions.com/t26231-backscatter+angle+measurement.html please could someone explain why - or point me in the direction of the information - why an angle of 173 degrees is used to measure backscatter in a DLS monitor. I have found information that explains that (some) equipement uses 90 degrees and suggests that this is historical.
thank you, Jackie]]> Tue, 12 Mar 2013 06:56:39 GMT http://www.scientistsolutions.com/t26230-dynamic+light+scattering.html please could someone explain why - or point me in the direction of the information - why an angle of 173 degrees is used to measure backscatter in a DLS monitor. I have found information that explains that (some) equipement uses 90 degrees and suggests that this is historical.
thnak you, Jackie]]> Tue, 12 Mar 2013 06:36:31 GMT http://www.scientistsolutions.com/t25110-leica+dfc290+microscope.html We have a serious problem about the adjusting white balance of our Leica DFC290 microscope. We couldn't get clear images. How can we solve this problem?
Thanks in advance.]]> Wed, 12 Dec 2012 04:20:19 GMT http://www.scientistsolutions.com/t23694-trouble+shooting.html I have a problem that cells on microscope image aggregate side of slide and no cell image on the middle of slide. What can i do for this proplem. could you please give me information about this proplem, thanks.]]> Wed, 01 Aug 2012 05:49:03 GMT http://www.scientistsolutions.com/t23496-can+someone+tell+me+what+these+things+are_.html https://plus.google.com/photos/115469570037453054737/albums/5760852557325973025?authkey=CLC_w6rK-svpOQ
if you can tell me anything about them, just comment on the pic.
I purchased a storage locker, with a bunch of these types of items. Any help? Thanks.]]> Wed, 11 Jul 2012 21:52:48 GMT http://www.scientistsolutions.com/t23032-double+immunofluorescence.html 1. Mouse monoclonal antibody raised against the antigen of rabbit origin............Sec Antibody :- Goat anti-mouse IgG FITC
2. Goat polyclonal antibody raised against a ntigen of mouse origin.............Sec antibody :- Rabbit anti-goat IgG Cy3
My query is that can I use these antibody combinations for doing double antibody staining in Immunofluorescence without the antibodies cross reacting with each other.]]> Sat, 05 May 2012 01:27:47 GMT http://www.scientistsolutions.com/t22920-advanced+microscopy+course_+amsterdam%2c+11_15+june+2012.html This practical advanced course will provide students the theoretical background and give hands-on experience of state-of-the-art (membrane) microscopy techniques
The program includes:
- Lectures
- Student presentations
- Hands-on practical sessions
- Networking and social program
The course is organised by the van Leeuwenhoek Centre for Advanced Microscopy and the European Science Foundation and focuses on membrane microscopy....]]> Fri, 20 Apr 2012 22:31:49 GMT http://www.scientistsolutions.com/t21839-how+to+quantify+fluorescence++intensity+with+image+j_+please+help.html Hi there,
I was wondering if anyone can tell me how to quantify fluorescence intensity with image J. I don't need to analyze single cells, I just want to measure the fluorescent intensity of an image (or selected area of an image) and compare with others taken under same conditions.
According to the protocol I know that I have to do the following:



Convert the color picture to 8it-b black and white scale.


Subtract the backg...]]> Fri, 27 Jan 2012 04:48:59 GMT http://www.scientistsolutions.com/t21240-image+j+for+histology+sesctions.html anyone can help me with the image j software to measure bone parameters like Bonevolume/tissue volume(BV/TV) in histologically stained sections.please do provide me th protocols for doing so
]]> Thu, 24 Nov 2011 01:47:16 GMT http://www.scientistsolutions.com/t19902-immunofluorescence+help.html Wed, 10 Aug 2011 11:34:11 GMT http://www.scientistsolutions.com/t19615-nucleus+stains+instead+of+actin.html I am staining the actin with phalloidin but the nucleus is staining. In some places, I do see some spots areound the nucleus.
Which normally does not occur. I would appreciate any help in this matter as to why the nucleus is getting stained rather than the actin!
Thank you
JJ]]> Mon, 18 Jul 2011 09:12:21 GMT http://www.scientistsolutions.com/t19472-mfm+with+the+veeco+cpii.html ]]> Thu, 07 Jul 2011 05:28:55 GMT http://www.scientistsolutions.com/t19407-how+to+quantify+dead+and+live+cellsusing+imajej.html I have got some images of Insulinoma cells , which have been stained with propidium iodide and hoechest to study the cell viability, I would be happy if someone could help me in quantify the Cell Viability using Fluroscent microscopy image by using ImageJ software, my Adavance thanks for your kind help
Farooq]]> Wed, 29 Jun 2011 09:37:51 GMT http://www.scientistsolutions.com/t18672-imagej+for+mitochondrial+movement.html Sun, 15 May 2011 18:52:31 GMT http://www.scientistsolutions.com/t18514-confocal+microscopy+image+drift.html I've got some confocal images that I'm trying to stack, but there was some X-Y drift between each optical slice, so the 3D image ends up a bit blurred. Does anyone know of any software I can use to minimise this drift? I'm currently using ImageJ for my image processing, so if you can recommend a plugin that would be great!
Thanks]]> Mon, 02 May 2011 05:19:18 GMT http://www.scientistsolutions.com/t18360-transferrin.html I hope to find somebody that have a protocol for Alexa Fluor® 568 conjugate transferrin. I have some problems with which dilution I have to use!
Thanks and happy Easter!
]]> Tue, 19 Apr 2011 10:27:26 GMT http://www.scientistsolutions.com/t18357-background+because+of+glycerol.html Thanks
]]> Tue, 19 Apr 2011 10:16:46 GMT