http://www.scientistsolutions.com/f37-cellular+and+molecular+immunology.html CTL (cytotoxic T lymphocytes), macrophages, T cells, B cells, Helper T cells, Natural Killer Cells (NKT), complement, granulocytes, dendritic cells, antigen presentation, Memory B cells, Neutrophils, eosinophils, mast cells, innate immunity, phagocytosis, TH1, TH2, basophils, MHC, antigen presentation Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t26909-t+cell+proliferation+using+cfse.html Do anyone know what actually...]]> Mon, 13 May 2013 16:58:58 GMT http://www.scientistsolutions.com/t25541-lowptpn6_shp1+hyperactivates+innate+immune+sys+_amp%3b+bacterial+control+impaired.html ]]> Mon, 21 Jan 2013 12:47:02 GMT http://www.scientistsolutions.com/t25353-problems+with+regulatory+t+cells+characterization.html 1. I would like to ask you how I can characterize Tr1 cells, can I use only CD4 CD25 and IL10 ?
2. The second question is : Can I use only CD4+Foxp3+ to characterize natural Regulatory T cells ?
Best reguards]]> Sun, 30 Dec 2012 01:40:14 GMT http://www.scientistsolutions.com/t22033-immunoglobulin+family.html kindly guide me regarding immunoglobulin family search.
as per my knowledge kabat database is useful to find out vh and vl gene family.
Thanks]]> Sun, 19 Feb 2012 06:32:25 GMT http://www.scientistsolutions.com/t21421-interferon_mediated+antiviral+response_+two+kinases+in+fish.html ]]> Wed, 14 Dec 2011 13:22:09 GMT http://www.scientistsolutions.com/t21214-lyn+is+a+redox+sensor+that+mediates+leukocyte+wound+attraction+in+vivo.html Mon, 21 Nov 2011 13:38:17 GMT http://www.scientistsolutions.com/t20189-immunohematology.html 2. How to inhibit serum clotting prior to antibody screening.]]> Sat, 27 Aug 2011 03:43:00 GMT http://www.scientistsolutions.com/t20133-antibody+treatment+for+infectious+disease+vs+reccurence%2c+how+does+it+work_.html Thanks
Chris
]]> Tue, 23 Aug 2011 14:29:19 GMT http://www.scientistsolutions.com/t19850-isolating+stromal+vascular+fraction+from+human+adipose+tissue.html at the moment I'm trying to optimize my protocol for the isolation of stromal vascular fraction from human adipose tissue for flow cytometry measurements. According to my protocol I first wash the tissue with PBS, cut it into 1-2 mm pieces and digest with 1 mg/ml collagenase in krebs-ringer-bicarbonate buffer for 40 minutes at 37°C. After the digestion I filtrate the cells first through 100 µm and 40 µm (this step takes longer if I have more tissu...]]> Tue, 02 Aug 2011 08:11:01 GMT http://www.scientistsolutions.com/t19148-zebrafish+embryo+defense+against+s_+pneumoniae+req_s+leukosyte+phagocytosis.html ]]> Mon, 13 Jun 2011 11:44:26 GMT http://www.scientistsolutions.com/t18345-nature+webcast_+lineage+differentiation.html

Current Controversies:
Lineage Differentiation

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Date:
April 19, 2011
Time:
12:00 PM EST / 9:00 AM PST ...]]> Mon, 18 Apr 2011 13:33:49 GMT http://www.scientistsolutions.com/t17583-carp+sitr+is+involved+in+the+nitric+oxide_mediated+response+to+a+protozoan.html Mon, 07 Feb 2011 18:40:17 GMT http://www.scientistsolutions.com/t17580-intravital+imaging+of+macrophage+_amp%3b+neutrophil+behaviour+during+inflammation.html ]]> Mon, 07 Feb 2011 14:48:55 GMT http://www.scientistsolutions.com/t17037-potential+of+antisense+morpholinos+for+primary+immunodeficiency+diseases.html ]]> Wed, 15 Dec 2010 14:21:45 GMT http://www.scientistsolutions.com/t16978-sperm.html ]]> Wed, 08 Dec 2010 06:00:47 GMT http://www.scientistsolutions.com/t16747-problems+inducing+thioglycollate+peritonitis.html I have been having problems inducing thioglycollate-induced peritonitis in mice. The purpose of the experiment is to observe migration defects in peripheral macrophages. Unfortunately, I have had inconsistencies in my FACS data (staining for F4/80) that haven't gone away. I have gone from observing a 12-20% increase in infiltrating macrophages (F4/80 dim) to 2-6%. I was wondering if anyone could help me fix the protocol so a greater difference can be...]]> Wed, 17 Nov 2010 14:25:34 GMT http://www.scientistsolutions.com/t16733-mouse+macrophage+phagocytosis+assay.html A few questions if you can help:
How many mice will I need to start with?
How many peritoneal cells do we expect per mouse?
At what speed / temperature / time should I centrifuge the mouse peritoneal cells to isolate the macrophages?
In the phagocytosis assay, the final counting of macrophages with ingested erythrocytes, how do we count the cells? From the whole co...]]> Mon, 15 Nov 2010 05:01:17 GMT http://www.scientistsolutions.com/t16506-identification+of+mouse+peritoneal+macrophages.html Thank you a ton !]]> Mon, 01 Nov 2010 05:02:54 GMT http://www.scientistsolutions.com/t16265-need+help+for+searching+antibodies.html Antigen:CD36
mouse anti rat
Applications: Flow Cytometry
Antigen: CD42B
mouse anti rat
Applications: Flow Cytometry
Please contact with me if you know which company has these products. Thank you.
]]> Mon, 11 Oct 2010 22:35:49 GMT http://www.scientistsolutions.com/t15968-transcriptome+analysis+of+traf6+function+in+the+innate+immunity+of+zfish.html Wed, 22 Sep 2010 16:19:51 GMT