http://www.scientistsolutions.com/t23068-native+non_denaturing%2c+non_reducing+page+and+western+blotting.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t23068-native+non_denaturing%2c+non_reducing+page+and+western+blotting.html As per the information, recommendation is to opt for native PAGE. You can refer to any manual for the protocol.
Blue Native PAGE is different from native PAGE and samples are treated with CBB 250 (G o R) prior to native PAGE. It is primarily used to detect protein interactions and formation of protein complexes.
]]> Tue, 08 May 2012 05:03:02 GMT http://www.scientistsolutions.com/t23068-native+non_denaturing%2c+non_reducing+page+and+western+blotting.html I am new to protein analysis and I am getting rather confused and was hoping someone can help please.
I would like to do a Western Blot to quantify the amount of collagen I have in some cells. I have an Abcam antibody already that I have successfully used for IHC, but according to the antibody datasheet I need to use non-denaturing and non-reducing conditions for westerns.
From what I've read, I need to use Native-PAGE, and not use any detergents (SDS, Triton), and not boil th...]]> Tue, 08 May 2012 03:41:09 GMT