http://www.scientistsolutions.com/c204-genomics.html Discussion on methodology for high-throughput genome scale analysis including next-generation sequencing, gene expression, SNP genotyping, CGH, bioinformatic approaches and useful software for genomic testing… Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t25156-simultaneous+rna+and+dna+extraction.html I want to isolate RNA and DNA from Anopheles mosquito samples. I have been trying different variations of the TRIzol protocol (back extraction buffer, sodium citrate) and have had little success in recovering good gDNA. My 260/230 ratios have generally been poor (0.30-0.60) and the absorbance peak at 270 nm! What's more puzzling is that when run on the gel, I get no bands!
I know there are some kits out there that can be a better alternative but I was wondering if any...]]> Fri, 14 Dec 2012 05:05:00 GMT http://www.scientistsolutions.com/t24989-genomics+careers+in+the+u_s_.html HireLifeScience.com is an employment resource and networking tool for the Pharmaceutical, Biotechnology, Medical Device and Life Science industries. We work exclusively with direct employers only and no third party recruiters giving you dir...]]> Wed, 05 Dec 2012 06:33:31 GMT http://www.scientistsolutions.com/t23500-sequencing.html My main aim is to detect the microbial diversity in a given sample by amplifying the variable regions of different microbes.
I used to amplify variable regions and get a 340 bp product and with this product i used to clone it by topo vector and later on used to sequence each single clone by sanger sequencing and the sequences were very fine.
But when i gave the ...]]> Fri, 13 Jul 2012 00:16:12 GMT http://www.scientistsolutions.com/t21996-dna+extraction+of+adipose+tissue.html Thanks in advance!!!]]> Tue, 14 Feb 2012 02:52:12 GMT http://www.scientistsolutions.com/t20785-need+help+finding+loci+for+genotyping+assay.html ]]> Thu, 13 Oct 2011 00:59:34 GMT http://www.scientistsolutions.com/t20013-ngs+data+analysis+learning+program.html Bionivid is conducting NGS data analysis learning program at Bionivid's Genome-Informatics Learning Centre at Bangalore...For more details please visit the website or facebook profile
http://www.bionivid.com/learning.html
https://www.facebook.com/Bionivid
]]> Wed, 17 Aug 2011 03:22:30 GMT http://www.scientistsolutions.com/t19155-genomics.html Thanks
Annie]]> Wed, 15 Jun 2011 02:57:42 GMT http://www.scientistsolutions.com/t19005-rapid+sequencing+enables+analysis+of+german+e_+coli+ourbreak.html This article from Genomeweb is also very informative. A quote in this article from Alexander Mellmann, a scientist involved in this research, demonstrates the importance of rapid sequencing: ""Speed is v...]]> Mon, 06 Jun 2011 00:02:07 GMT http://www.scientistsolutions.com/t18614-transcriptome+sequencing+of+an+unknown+genome.html Tue, 10 May 2011 13:26:24 GMT http://www.scientistsolutions.com/t18382-no+colonies+after+lr+recombination.html ]]> Wed, 20 Apr 2011 22:30:09 GMT http://www.scientistsolutions.com/t18220-join+online+meeting+_+mechanism+of+dna+damage+and+repair+for+free.html ]]> Thu, 31 Mar 2011 16:47:12 GMT http://www.scientistsolutions.com/t17373-hdac1+promotes+transcription+of+core+neurogenic+programme+in+zfish+embryos.html Mon, 17 Jan 2011 15:43:21 GMT http://www.scientistsolutions.com/t17214-synthtic+gene+design.html I am trying to design a syntheic gene which has to be codon optimised for tomato plant.
please tell me is there any software or program to design the gene. steps need to be taken care in designing .
thank u in advance
]]> Mon, 03 Jan 2011 02:58:34 GMT http://www.scientistsolutions.com/t17014-opinions+wanted+for+an+anti_cancer+thesis+please.html I am a master degree student and am interested to do my thesis in the field of cancer, especially in the area of cancer and drug resistance. However, since the field is highly competitive in these days followed by like to find a research niche I came-up with a small hypothesis/plan, but certainly not sure whether it’s already established issues and missed to locate the right literatures or whether it might be worth to experiment further with the expensive techniques suc...]]> Fri, 10 Dec 2010 18:48:05 GMT http://www.scientistsolutions.com/t16914-determining+the+genomic+sequences+of+related+fungal+strains.html


Hello all,
So I was asked to come up with an sequencing plan that allows me to determine the genome sequences of
a fungal species that has various strains that are pathogenic to either 1 or 2 crops, but not three.
(in my case its wheat, barley and corn)
the catch is I cant use Sanger method since its so expensive/time consuming
Any ideas?
thank you,
A, from Montreal

]]> Wed, 01 Dec 2010 21:03:43 GMT http://www.scientistsolutions.com/t16740-illumina+gaiix+available+to+a+good+home_.html interested. I am looking to upgrade to Hiseq, or if anyone has an abuncance of ABI 3730xls we can talk about a trade.
]]> Tue, 16 Nov 2010 13:07:41 GMT http://www.scientistsolutions.com/t16135-comparison+of+genome+sequences.html Tue, 05 Oct 2010 20:54:06 GMT http://www.scientistsolutions.com/t15661-please+explain.html i know tht NAT2 is a gene coding for a protein but i've not been able t figure out wat stands for ' * ' followed by a number(eg 5,here) and A???
can anyone please explain...
NAT2*4 is said to be wild type......i cant understand anything :((
please....]]> Mon, 23 Aug 2010 09:18:31 GMT http://www.scientistsolutions.com/t14845-the+non_coding+genome.html Mon, 28 Jun 2010 05:21:31 GMT http://www.scientistsolutions.com/t14794-transcripts+analysis.html I am working on in vitro expression analysis. I have transfected a mammalian cell line with a gene of wild type and mutant one. surprisingly, I found that the transcript level of the wild type gene was less than the mutant (which is against the logic theory where the wild type should be expressed more than the mutant)..... I wondered what is the reason behind the less expression of the wild type than the mutant one.... any idea because I got exhausted with this without finding an answe...]]> Fri, 18 Jun 2010 10:54:37 GMT