http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html Wed, 10 Apr 2013 04:14:54 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html Fri, 14 Oct 2011 04:53:37 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html ]]> Wed, 08 Jun 2011 13:22:46 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html http://www.nature.com/nrg/posters/regulation/index.html
]]> Thu, 30 Sep 2010 15:45:46 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html I would be very happy if any one explain the working mechanism of miRNA other than RNAi pathway ?

Thanks
KC]]> Thu, 30 Sep 2010 15:00:24 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html An siRNA knockdown without off-target effects would be an unlikely outcome.
Comparison of siRNA-induced off-target RNA and protein effects. Aleman LM, Doench J, Sharp PA. RNA. 2007 Jan 19; [Epub ahead of print]
Retraction of synapses and dendritic spines induced by ...]]> Fri, 23 Mar 2007 20:39:42 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html The use of the term siRNA for both exogenous (from synthetic sources) and endogenous (from long dsRNA) short dsRNA is somewhat confusing, but I think that it is so thoroughly established that it is unlikely a clearer nomenclature will quickly supplant the current usage. Another confusing aspect of this nomenclature is the experimental use of short hairpin RNA, which can be transfected as an oligo or expressed in the target cell from a plasmid. Is this a ...]]> Fri, 23 Mar 2007 16:44:18 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html The use of the term siRNA for both exogenous (from synthetic sources) and endogenous (from long dsRNA) short dsRNA is somewhat confusing, but I think that it is so thoroughly established that it is unlikely a clearer nomenclature will quickly supplant the current usage. Another confusing aspect of this nomenclature is the experimental use of short hairpin RNA, which can be transfected as an oligo or expressed in the target cell from a plasmid. Is this a subset of miRNA, o...]]> Tue, 20 Mar 2007 15:16:57 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html -----------------------
Nat Rev Mol Cell Biol. 2007 Jan;8(1):23-36.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17183358&query_hl=12&itool=pubmed_DocSum
"In addition to endogenous microRNA...]]> Mon, 19 Mar 2007 21:16:58 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html I heard this description at a developmental bio meeting and it seems to fit common usage.
siRNA is a synthetic molecule, consisting of double-stranded RNA with short single-stranded ends, which is transfected into cells and binds to RISC. siRNA is perfectly complementary to a particular target mRNA and cleaves that target. siRNA also suppresses translation of many off-target mRNAs that have partial sequence complementarity. The siRNA molecules may be indistinguishable from th...]]> Fri, 16 Mar 2007 15:38:11 GMT http://www.scientistsolutions.com/t3938-what+is+the+difference+between+mirna+and+sirna_.html Thu, 15 Mar 2007 04:10:05 GMT