http://www.scientistsolutions.com/t22964-calculated+vs+measured+ljp.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t22964-calculated+vs+measured+ljp.html I still don't understand why my agar bridges did not seem to work correctly.]]> Thu, 16 May 2013 09:50:25 GMT http://www.scientistsolutions.com/t22964-calculated+vs+measured+ljp.html I used JPCalc to calculate the liquid potential for my experiments, in order to verify the result I measured the liquid junction potential as well.
The problem that occurs is that when I change my aCSF bath solution for my internal solution the potential I measured before does not reverse, which it should.
Can somebody give me some advice on this?
My measurement procedure is as follows:
I start with internal solution i...]]> Tue, 14 May 2013 05:29:23 GMT http://www.scientistsolutions.com/t22964-calculated+vs+measured+ljp.html Would you provide the composition of both intra and bath solutions? I understand Igor is the software, not program.
How you measured the LJP? By changing the bath solution to the another one? Do you have the agar bridge between recording chamber and the reference electrode?
Thanks.]]> Thu, 26 Apr 2012 16:05:06 GMT http://www.scientistsolutions.com/t22964-calculated+vs+measured+ljp.html Please advise on which numbers I should use to correct my membrane voltage, and what could be causing such a large discrepancy ...]]> Thu, 26 Apr 2012 13:56:53 GMT