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<title>How to do a kinetic assay measuring NADH</title>
<link>http://www.scientistsolutions.com/t20697-how+to+do+a+kinetic+assay+measuring+nadh.html</link>
<description> Life Science Discussion</description>
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<title>How to do a kinetic assay measuring NADH</title>
<link>http://www.scientistsolutions.com/t20697-how+to+do+a+kinetic+assay+measuring+nadh.html</link>
<description><![CDATA[Hi Jius,<br />Yes, you need to have a standard curve of NADH so that you can convert the amount of NADH and its absorbance for your calculation. Also, you need to know how much protein you add to your reaction.<br />Cheers.<br />]]></description>
<pubDate>Thu, 06 Oct 2011 10:35:45 GMT</pubDate>
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<item>
<title>How to do a kinetic assay measuring NADH</title>
<link>http://www.scientistsolutions.com/t20697-how+to+do+a+kinetic+assay+measuring+nadh.html</link>
<description><![CDATA[Hi, i am trying to measure the activity of an enzyme called alanine  aminotransferase in plant tissue (sorghum shoots and roots).  The assay i  am using monitors NADH oxidation: when enzyme activity is higher, NADH  is oxidized, so its concentration in the reaction decreases.  I have  done a kinetic assay and obtained the delta absorbance value (dA/min) at  340 nm (5 minutes total, 9 second intervals); my question is: how do i  convert the dA/min value to micromol/ microgram protein/ m...]]></description>
<pubDate>Wed, 05 Oct 2011 21:13:35 GMT</pubDate>
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