http://www.scientistsolutions.com/c202-antibody+based+technologies.html Discussion on antibodies techniques including polyclonal, monoclonal, peptide design, antisera, labeling, ELISA, fusion proteins, complement activation, isotypes, Fab, Fc, class switching… Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t26934-osteoblasts+specific+antibodies+for+western+blot.html Could any one suggest me some good working antibodies for osteoblasts specific markers such as Osteocalcin, BGLAP, Osteopontinand Dickkopf 1 ??
Thanks in advance
]]> Fri, 17 May 2013 05:39:27 GMT http://www.scientistsolutions.com/t26735-atcc+5c8+supernatant+storage+_.html i'm asking if there is someone working with the atcc 5c8 CELL LINE (cat. num. HB-10916), in fact iwant to know if it's possible to storage the supernatant (used as anti CD154) and in under which conditions?
thank you ]]> Wed, 01 May 2013 06:08:51 GMT http://www.scientistsolutions.com/t26582-phalloidin+and+methanol.html I'm facing a well known problem with phalloidin: it doesn't work with a fixation in Methanol.
My issue is that the only antibodies i have found for my protein of interest and working in immunocytochemistery are working with the fixation in Methanol.
Somebody told me that i should try to start with a PAF fixation, and then 5 minutes with Methanol.
What do you think about this? I'm working on mouse primary cortical neurons.
Thank you very much,
Rachel...]]> Wed, 10 Apr 2013 02:38:20 GMT http://www.scientistsolutions.com/t26159-antibodies+for+facs+and+fluorescence+microscopy.html thanks ]]> Thu, 07 Mar 2013 01:54:29 GMT http://www.scientistsolutions.com/t24882-inter_assay+od+variation.html Next day we block and add rabbit anti-peptide and then anti-rabbit HRP and measure signal. All steps are optimised and controls are good (inter-precision 10% or less, intra precision 4%).
But the OD of the standard curve shifts a lot. - %CV of top standard OD is 20%. (Excluding extreme three ...]]> Wed, 28 Nov 2012 06:29:20 GMT http://www.scientistsolutions.com/t24564-wbcs.html ]]> Tue, 23 Oct 2012 13:00:12 GMT http://www.scientistsolutions.com/t24563-wbcs.html ]]> Tue, 23 Oct 2012 12:57:44 GMT http://www.scientistsolutions.com/t24562-hiv_aids+does+have+a+cure.html Mon, 22 Oct 2012 11:09:58 GMT http://www.scientistsolutions.com/t24560-western+blotting.html In western blotting first we do sds page na that time all the proteins will be linearised..
But after that wen we MAbs (which can recognise conformational and linear epitopes)to identify binds to the linearised protein how? that time as such there is no
conformational epitope na then how it binds...
wat is the mechanism behind it....
Thank you...]]> Sat, 20 Oct 2012 05:59:25 GMT http://www.scientistsolutions.com/t24501-where+do+you+buy+antibodies_.html Can you tell me on which website you go when looking for a primary antibody? a secondary antibody?
Thank you very much!
]]> Thu, 11 Oct 2012 07:17:04 GMT http://www.scientistsolutions.com/t24117-antibodies.html I have only idea that Antibodies are produced by a type of white blood cell called a plasma cell. Antibodies can occur in two physical forms, a soluble form that is secreted from the cell, and a membrane-bound form that is attached to the surface of a B cell and is referred to as the B cell receptor (BCR). Some antibodies act by binding to certain essential enzymes in the target bacteria. So, I want to know that how might these antibiotics influence the chemical reactions controlle...]]> Thu, 13 Sep 2012 21:33:13 GMT http://www.scientistsolutions.com/t24097-how+long+do+mold+antibodies+stay+in+your+blood+stream_.html My sister was getting allergy tested next Tuesday because of severe reaction to some kind of mold in her apartment. I read somewhere that mold antibodies produced from an allergy stay in your stream for around 30 days, but that was the only information I could really find. So, now I am in doubt. Any one having knowledge please suggest me.
Thanks!
Stephen Jones ]]> Thu, 06 Sep 2012 21:51:35 GMT http://www.scientistsolutions.com/t23625-prp+antibodies+specific+to+prpc+or+prpsc.html I have been looking the past few days for antibodies to recognize the native PrPc and PrPsc of mouse.
So far, I think I have found some good candidates, but I am wondering if anyone here is a prion specialist and might be able to help me out with options.
I need an Ab that will recognize both PrPc and PrPsc in their native states - without denaturing or Proteinase
K.
Also, an Ab that will recognize ONY PrPc in int native state.
An...]]> Mon, 23 Jul 2012 13:12:14 GMT http://www.scientistsolutions.com/t23445-protein+expression_.html Dear All,
I'm trying to get the soluble expression of Fab(Fragment of Antibody) in cytoplasma of E.coli.I have gone through the no of litratures and tried some experiment but not getting enough expression.
Actually my question is that ,What we can do as a upstream member (fermentation) to get the good level of soluble expression in e.coli?
I have tried lower temprature,lower agitation,low level of inducer concentration and used some modified media also to get the solu...]]> Thu, 05 Jul 2012 09:46:10 GMT http://www.scientistsolutions.com/t23407-affinity+purification+of+chicken+antibodies.html Thu, 21 Jun 2012 10:11:56 GMT http://www.scientistsolutions.com/t22738-dab+destain%2c+is+this+possible_.html Fri, 06 Apr 2012 09:27:46 GMT http://www.scientistsolutions.com/t21055-how+to+separate+a_g+beads+from+the+attached+antibodies_.html Thanks
VS
]]> Fri, 04 Nov 2011 12:16:57 GMT http://www.scientistsolutions.com/t20601-elisa+validation.html Does anyone have a protocol for immunoassays validation as I can't find clear guidelines for this??
or at least if anybody can provide me with any guidelines for the validation of immunoassays.
thank you]]> Wed, 28 Sep 2011 05:20:53 GMT http://www.scientistsolutions.com/t20485-elisa%2c+what+is+the+best+fit+for+the+standard+curve_.html Help! :(
I'm working on ELISA, using ELISA kit ready coated, ELISA reader with software.
I make dilution series to create standard curve (1000 pg/ml, 500, 250, 125, 62, 31, 15) but sometimes I apply in the reaction only 5 std.
the cytokine i'm working with has very low level in human serum, almost around the lowest std. (15 pg/ml)
I got std. curve with R^2 > 0.99 which is very good but still not enough, because I need almost perfect std. curv...]]> Mon, 19 Sep 2011 05:44:10 GMT http://www.scientistsolutions.com/t20098-are+iggs+secreted+in+the+gut_.html I was reading a paper about a phase II clinical trial involving IV injection of monoclonal anti-toxin IgGs againt C. difficile toxine A and B. I thought that only IgA were secreted in the gut... How can an IV of IgG be effective against a bug in the gut?
(Leav et al 2010 Vaccine 28 (2010) 965–969)
Thanks
]]> Mon, 22 Aug 2011 17:14:45 GMT