western blot problem http://www.scientistsolutions.com/t23489-western+blot+problem.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 western blot problem http://www.scientistsolutions.com/t23489-western+blot+problem.html There can be multiple reasons or it:
1) you are using a membrane wit high pore size and transferred it for long duration leading to escape of your LMW proteins
2) A bubble or air space between your gel and membrane
3) Ponceau has alot lower sensitivity in comparison to CBB g250 and as a result youre unable to detect proteins at this range. Increase the loading amount and you may be able to visualize them.
4) degraded proteins in LMW region ]]> Wed, 11 Jul 2012 05:33:09 GMT western blot problem http://www.scientistsolutions.com/t23489-western+blot+problem.html I did western today and notice that I saw only 5 (from 6 ) - 50 kd bands of the marker.
in addition, in the ponceau I saw bands only untill the 50 kd marker.
meaning, I dont see protiens that are smaller than 50kd.
what could be the problem?
Shani
]]> Wed, 11 Jul 2012 05:21:53 GMT