http://www.scientistsolutions.com/t16106-egfp+fusion+proteins.html Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t16106-egfp+fusion+proteins.html Sun, 03 Oct 2010 22:25:50 GMT http://www.scientistsolutions.com/t16106-egfp+fusion+proteins.html Whether your protein prefers a fusion at the C- or N- terminus is largely case-dependent. But here are some questions you can ask yourself before coming up with a conclusion-
Do you know any structural information on this protein? If not, based of the primary sequence, what kind of info you can get? Does it has a specific function found at either the C- or N- terminus that introducing a fusion might kill that specific function? Take an enzyme with a cataly...]]> Sun, 03 Oct 2010 11:49:42 GMT http://www.scientistsolutions.com/t16106-egfp+fusion+proteins.html Sat, 02 Oct 2010 07:36:07 GMT