Glycobiology Protocol http://www.scientistsolutions.com/a595-protocolslist-glycobiology.aspx Webforum for Glycobiology - Discussion of glycans and their functions. Topics include glycan diversity, glycosyltransferases and processing enzymes, lectins, glycomics, glycan structure analysis, O-GlcNAc, methods in glycobiology research Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 Deglycosylatio using TFMS http://www.scientistsolutions.com/a7373-protocol-deglycosylatio+using+tfms.aspx
Glycosylation is one of the most common posttranslational modifications of proteins. Glycans attach to the peptide backbone through either amide or glycosidic bonds (N-linked or O-linked) and may be removed either enzymatically or chemically. Enzymatic modes offer mild conditions, but are specific for either N- or O-linked glycans. Chemical methods though are non-specific, but capable of removing the entire glycan complement under appropriate reaction co...]]> Wed, 06 Jul 2011 10:28:50 GMT Microplate PAHBAH Assay for Carbohydrate Reducing Ends http://www.scientistsolutions.com/a7288-protocol-microplate+pahbah+assay+for+carbohydrate+reducing+ends.aspx Reagent A: Slurry 1.0 g of p-Hydroxy benzoic acid hydrazide (PAHBAH) in 6.0 ml of ddH2O. To this, add 1 ml of concentrated HCl. Make up the volume to 20 ml with ddH2O.
Reagent B: Dissolve 2.49 g of trisodium citrate in 50 ml of ddH2O. To this, dissolve 0.22 g of calcium chloride or 0.292 g of calcium chloride trihydrate. To this, dissolve 4.0 g sodium hydroxide. Adjust the volume to 200 ml with ddH2O.
...]]> Mon, 17 May 2010 09:41:15 GMT Microplate Phenol-Sulfuric Acid Assay for Total Carbohydrate http://www.scientistsolutions.com/a7245-protocol-microplate+phenol_sulfuric+acid+assay+for+total+carbohydrate.aspx (1) Pipet 100 ul of concentrated sulfuric acid to a microplate. Note: Use acid resistant plate and pipette.
(2) Pipet 30 ul of ice-cooled sample to a sulfuric acid well. Note: Cooling the sample prevents violent boiling when aqueous sample is added to the sulfuric acid, it also increases the sensitivity and reproducibility of the assay.
(3) Pipe...]]> Fri, 30 Oct 2009 12:09:36 GMT Radioactive Glycosaminoglycan Labeling Protocol http://www.scientistsolutions.com/a7132-protocol-radioactive+glycosaminoglycan+labeling+protocol.aspx 2. Culture the cells for 2-3 days
The fol...]]> Tue, 04 Aug 2009 18:35:48 GMT Glycoproteomics Using Chemical Immobilization http://www.scientistsolutions.com/a6774-protocol-glycoproteomics+using+chemical+immobilization.aspx Mon, 06 Jul 2009 19:19:43 GMT Use of Glycosidases to Study Protein Trafficking http://www.scientistsolutions.com/a6767-protocol-use+of+glycosidases+to+study+protein+trafficking.aspx Mon, 06 Jul 2009 19:19:43 GMT Lectin Affinity Chromatography http://www.scientistsolutions.com/a6778-protocol-lectin+affinity+chromatography.aspx Mon, 06 Jul 2009 19:19:43 GMT Protocol for Beta-Elimination for Release of O-GalNAc-Linked Oligosaccharides from Glycoproteins and Glycopeptides http://www.scientistsolutions.com/a6772-protocol-protocol+for+beta_elimination+for+release+of+o_galnac_linked+oligosaccharides+from+glycoproteins+and.aspx Mon, 06 Jul 2009 19:19:43 GMT Beta-Elimination for Release of O-Linked Glycosaminoglycans from Proteoglycans http://www.scientistsolutions.com/a6773-protocol-beta_elimination+for+release+of+o_linked+glycosaminoglycans+from+proteoglycans.aspx Mon, 06 Jul 2009 19:19:43 GMT Sialidases http://www.scientistsolutions.com/a6754-protocol-sialidases.aspx Mon, 06 Jul 2009 19:19:42 GMT Special Considerations for Glycolipids and Their Purification http://www.scientistsolutions.com/a6757-protocol-special+considerations+for+glycolipids+and+their+purification.aspx Mon, 06 Jul 2009 19:19:42 GMT Special Considerations for Proteoglycans and Glycosaminoglycans and Their Purification http://www.scientistsolutions.com/a6759-protocol-special+considerations+for+proteoglycans+and+glycosaminoglycans+and+their+purification.aspx Mon, 06 Jul 2009 19:19:42 GMT Glycoconjugate Analysis http://www.scientistsolutions.com/a6724-protocol-glycoconjugate+analysis.aspx Abstract - Whereas DNA, RNA, and proteins are linear polymers that can usually be directly sequenced, oligosaccharides show substantially more complexity,having branching and anomeric configurations ( and linkages). The biosynthesis of oligosaccharides, termed glycosylation, is extremely complex, is not template-driven, varies among different cell types, and cannot be easily...]]> Mon, 06 Jul 2009 19:19:41 GMT Metabolic Radiolabeling of Animal Cell Glycoconjugates http://www.scientistsolutions.com/a6718-protocol-metabolic+radiolabeling+of+animal+cell+glycoconjugates.aspx Mon, 06 Jul 2009 19:19:41 GMT Inhibition of Glycolipid Biosynthesis http://www.scientistsolutions.com/a6704-protocol-inhibition+of+glycolipid+biosynthesis.aspx Mon, 06 Jul 2009 19:19:41 GMT Metabolic Labeling of Glycoproteins with Radioactive Sugars http://www.scientistsolutions.com/a6713-protocol-metabolic+labeling+of+glycoproteins+with+radioactive+sugars.aspx Mon, 06 Jul 2009 19:19:41 GMT Inhibition of N-Linked Glycosylation http://www.scientistsolutions.com/a6705-protocol-inhibition+of+n_linked+glycosylation.aspx Mon, 06 Jul 2009 19:19:41 GMT Endoglycosidase and Glycoamidase Release of N-Linked Glycans http://www.scientistsolutions.com/a6685-protocol-endoglycosidase+and+glycoamidase+release+of+n_linked+glycans.aspx Mon, 06 Jul 2009 19:19:40 GMT Determining the Structure of Glycan Moieties by Mass Spectrometry http://www.scientistsolutions.com/a6681-protocol-determining+the+structure+of+glycan+moieties+by+mass+spectrometry.aspx Mon, 06 Jul 2009 19:19:40 GMT Endo-Galactosidases and Keratanase http://www.scientistsolutions.com/a6687-protocol-endo_galactosidases+and+keratanase.aspx Mon, 06 Jul 2009 19:19:40 GMT