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Introduction The Adeno-3™ Expression System provides an efficient method for constructing recombinant adenoviruses. Our procedure uses in vitro ligation to subclone your gene of interest in
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The Adeno-2™ Expression System provides reagents for the efficient construction of recombinant adenoviral vectors with your expression cassettes being HA or His tagged, facilitating your t
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The Adeno-1™ Expression System provides an efficient method for constructing recombinant adenoviruses. Our procedure uses in vitro ligation to subclone your gene of interest into a replicati
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Adenoviral Vector Amplification Most adenoviral vectors are supplied as a 250ul seed stock. With this stock, customers can produce as much virus stock as they desire. Because of this feature, it is
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Introduction It has been well-documented that primary cells only undergo a pre-determined and finite number of cell divisions in culture (Stewart SA. 2002). After limited population doublings (th
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This protocol provides provides detailed procedure for the generation of glutathione-coupled beads for flow cytometry experiments with cells expression GST-fusion proteins. Normal
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Size-exclusion chromatography (SEC) is a popular method to separate biomolecules based on their size. Primarily, it is applied to the separation of biopolymers such as proteins and nucleic acids, i.e.
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  Many biochemical processes are markedly impaired by even small changes in the concentrations of free H+ ions. It is therefore usually necessary to stabilize the H+ concentration in vitro by ad
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Advanced experiments in gene technology demonstrate that even small amounts of free DNA molecules are sufficient to cause infections, recombination or biological transformation. The complete decontami
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RNA coimmunoprecipitation (co-IP) experiments are an extension of protein co-IP experiments in which in vivo RNA-protein complexes are investigated. This protocol describes how to perform RNA co-IPs f
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