Homologous recombination is an important mechanism for the repair of damaged chromosomes, for preventing the demise of damaged replication forks, and for several other aspects of chromosome metabolism
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In Drosophila melanogaster, as in many other animals, courtship is a series of stereotypical behaviors carried out by a male responding to multimodal signals. Because different experimental conditions
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This article provides a general introduction to keeping Drosophila stocks, making and scoring crosses, mutagenesis, and controlling diseases in the laboratory.
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This protocol describes the loading of individual cells with fluorescent probes via patch pipettes. The patch-clamp methodology has been successfully used for single-cell dye labeling in cultured neur
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Protein-DNA interactions (PDIs) between transcription factors (TFs) and their target genes form the backbone of transcription regulatory networks. Such PDIs can be identified with either a TF or a gen
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The following protocol describes the synthesis of short linear peptides, or peptide pools, on modified cellulose membranes, and the detection of their protein-binding partners. Peptides are synthesize
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This protocol describes the use of methylated CpG island amplification (MCA) in combination with a microarray platform to analyze genome-wide DNA methylation in a high-throughput fashion. In this appr
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The majority of mouse chromosome preparations for banding are now made by air-drying and, in essence, require the production of a cell suspension as a starting point. Some samples such as blood cultur
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Systematic mutational analysis is required for the comprehensive deciphering of gene function. However, repeated targeting of a single locus is labor intensive and has not been a routine approach for
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A breakthrough in Arabidopsis research was the invention of the vacuum-infiltration procedure, a simple and reliable method of obtaining transformants at high efficiency while avoiding the use of tiss
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