3D Fibroblasts Culture and Transfection Protocol This protocol is provided for a 96-well scaffold. 1- Dilute 0.5 μg of plasmid DNA in 17 μL of serum-free medium (DMEM, RPMI or other gr
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 Flow cytometry is a powerful technique for assessing the expression of multiple proteins simultaneously in individual cells within a heterogeneous population. This technique is most commonly use
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 Modern clinical drug development relies on both in vivo and ex vivo imaging to assist in lead qualification, efficacy assessments, toxicity determinations, and biomarker discovery and validation
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The patent pending ALL-TAIL™ Kit can be used to investigate the dynamic changes in poly(A) tail length that take place during gene expression, as well as the changes in 3’ untranslated reg
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The ExoMir™ kit is designed for the fractionation of, and RNA extraction from, exosomes and other microparticles in cell-free fluids. The ExoMir Kit uses rapid fractionation approach in which sa
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This protocol is a miniaturization of PAHBAH assay protocol from Megazyme. Reagent A: Slurry 1.0 g of p-Hydroxy benzoic acid hydrazide (PAHBAH) in 6.0 ml of ddH2O. To this, add 1 ml of concent
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 Sutter's Pipette Cookbook. A very useful read for all electrophysiologists
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 The eosinophil count is performed by diluting whole blood with a staining solution. The phyloxine B present in the diluting fluid stains only the eosinophils red; all other leukocytes are preser
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TUNEL assay (in situ detection of apoptotic cells) reference :Gavrieli Y, Sherman Y, Ben-Sasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentat
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Progesterone receptor/ BrdU double immunofluoresence
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