The baculovirus expression vector system has been used extensively to produce numerous proteins originating from both prokaryotic and eukaryotic sources. In addition to easy cloning techniques and
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Genome sequencing has identified a massive number of uncharacterized genes in Arabidopsis thaliana and several other plant species. To decipher these unknown gene functions, several transient expre
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Embryos of the frog Xenopus laevis are an ideal model system for in vivo imaging of dynamic biological processes, from the inner workings of individual cells to the reshaping of tissues during embr
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Vertebrate development is best studied in an intact embryo model, but a robust interface between time-lapse microscopy and in vivo embryo health and maintenance can be difficult to achieve in model
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The rapidly growing arsenal of genetically encoded fluorescent proteins (FPs) obtained from sea creatures has launched and fueled a revolution in live cell imaging. The diverse array of application
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Obtaining quantitative data from live cell images is the key to testing mechanistic hypotheses of molecular and cellular processes. The importance of using computer vision-based methods to accompli
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The giant fiber system (GFS) of Drosophila is a well-characterized neuronal circuit that mediates the escape response in the fly. It is one of the few adult neural circuits from which electrophysio
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The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells) must be competent to generate many thousands of diff
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  Make 1 liter each of two separate 20X solutions Solution 1  :  348 mM Na2HPO4      46.86g 70 mM Na2H2PO4      9.66g Solution 2:
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We usually transfect RAW264.7 in a 24 well-plate culture vessel and get around 50 % - 60 % of transfected cells. 1) Seed 50,000 RAW264.7 cells per well the day before transfection 2) Dilute 0.5-1
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