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deglycosylation of heavily glycosylated protein

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Rajiv Dua
India

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Posted By Rajiv Dua
on 11/20/2009 23:02 PM   
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Hi...i hope this article will be useful to You..

Deglycosylation of glycoproteins with trifluoromethanesulphonic acid: elucidation of molecular structure and function.
Albert S B Edge
Harvard Medical School and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA. Albert_Edge@meei.harvard.edu
 

With Regards,
Rajiv

Learning is a treasure that will follow its owner everywhere.....RD
Tegrion
Russia

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Posted By Tegrion
on 11/25/2009 0:35 AM   
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Hi!!

Thank you, Rajiv Dua! Very interesting article!

Brilliant thoughts!
R Bishop
United States

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Posted By R Bishop
on 11/25/2009 7:29 AM   
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Yeah nice.  I havent seen that one.  Thanks!

"Everything should be made as simple as possible, but no simpler." -- Einstein
Tegrion
Russia

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Posted By Tegrion
on 11/26/2009 8:45 AM   
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Dear colleagues!! What do you think, can alkylation of protein by acrylamide affects PTMs (glycosilation, particularly)? We could not explain phenomenon of decreasing of quantity of pI modification of protein of interest after alkylation. And i did not such information. Thank you for your suppositions!
Tegrion
Russia

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Posted By Tegrion
on 11/27/2009 3:26 AM   
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Hi!!

Our argue is endless. How do you think, can acrylamide react with -COOH during alkylation process? Or alkylation specific for -SH group only?

Thank you a lot!
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