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margot001
Member since: Jun 02, 2010
From: France
Status: Frog Egg
My points: 14    what's this
Name: [Privacy]
 


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My Activity Log    Sort Activities by Type
Jun 27, 2011    05:34 AM
Posted new protocol   Mouse Embryonic Stem Cells Transfection

Mouse Embryonic Stem Cells cultured on a matrigel-coated 100 mm dish have been transfected with GeneCellin HTC. We have used 2x106 cells in a 100 mm dish, 15 µg of plasmid DNA and 30 µL of GeneCellin HTC reagent. We have obtained about 50% of transfected embryonic stem cells ...
Jul 5, 2010    09:42 AM
Posted new protocol   Antibody delivery into living cells

We have delivered a fluorescent labeled IgG-DyLight into VERO cells 1- 1µg of IgG was dilute in 10 µL of PBS 2- 2 µL of ImmunoCellin reagent (BioCellChallenge SAS) was added 3- mixture was incubated 15 min at RT 4- then diluted with 90 µL of serum free medium and added ...
Jun 22, 2010    05:52 AM
Posted new protocol   RAW264.7 Cells Transfection

We usually transfect RAW264.7 in a 24 well-plate culture vessel and get around 50 % - 60 % of transfected cells. 1) Seed 50,000 RAW264.7 cells per well the day before transfection 2) Dilute 0.5-1 µg of DNA in 100 µL of serum free medium 3) Add 3 µL of GeneCellin transfection r ...
Jun 2, 2010    07:14 AM
Posted new protocol   3D Fibroblasts Culture and Transfection

3D Fibroblasts Culture and Transfection Protocol This protocol is provided for a 96-well scaffold. 1- Dilute 0.5 μg of plasmid DNA in 17 μL of serum-free medium (DMEM, RPMI or other growth medium). 2- Add 3 μL of 3D Transfection Reagent (BioCellChallenge SAS) to the diluted DNA ...




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