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Biju' Bench [how it works]
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Keeps a log of the topics you've started, the replies you've made, protocols or events you've posted, etc. Your summary will show up in your public profile.

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My Lab Drawer (private)
As you browse through Scientist Solutions, you may see a topic, event, job or protocol that you'd like to store so that you can find it again easily. By clicking the "Save to My Bench Space" button next to any item on our site, you will put that item in your lab drawer for future reference.
You can also add fellow scientists to your lab drawer by clicking the "Save this scientist to My Bench Space" link next to that member's name in their posts or Bench Space profiles.

My PubMed Alerts (private)
This really cool tool allows you to set up email notifications whenever and article in your area of interest (specified by keyword is published in PubMed

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Biju
Member since: Feb 06, 2007
From: Maryland, United States
Status: Frog Laureate
My points: 269    what's this
Name: Biju
 


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Aug 22, 2011    09:15 AM

Replied to topic in Virology forum   Vccine

Rajeswari Your question requires an answer that could not be covered in a small reply or even by email.The start point is finding the best epitopes . This needs quite big investment of time- in synthesizing compounds; carrying out mice experiements, modifying the compounds and so on before act ...

Jul 21, 2011    02:00 PM

Replied to topic in General forum   Starting Salary in USA

Micky mouse You should what are the deductions from your salary. 1.Does the salary include amount for your health insurance? 2. Is the health insurance is covered by institution and not by PI? 3. Is there tax deductions? Hope this helps. Biju

Jul 21, 2011    01:53 PM

Replied to topic in DNA (PCR, Real Time PCR) forum   no amplification

Priyanka Could you upload a gel of your genomic DNA and also 1 or 2 representative gel of your PCR which worked well before and also of the PCR with light smear? Biju

Jul 18, 2011    12:42 PM

Replied to topic in DNA (PCR, Real Time PCR) forum   VECTOR NTI

Hi Silver One reason for not getting any results in blast could be due to the long length og the cDNA sequence you have used.I suggest you input 150-200 bp sequence around which you want to design the primers.You should also try blastn instead of blast. Biju

Jun 2, 2011    08:23 PM

Replied to topic in DNA (PCR, Real Time PCR) forum   sybr safe

Hi I have seen a system similar to flash gel; but it is not a hand held sytem.But you could see the DNA band by turning on a switch when the DNA will be illuminated by sybrSafe(?I am not sure).The problems are 1.Yyou cannot use the band for Sequencing applications by fluorescent method 2. If y ...

May 26, 2011    08:29 AM

Replied to topic in RNA forum   RNA Levels

Hi Annie I have not isolated RNA from blood plasma and hence does not know about the yield. I will derive my own conclusions about the yield than depending on reported literature . I will start with fairly good amount of fresh blood plasma, process as early as possible and will quantify the RNA l ...

May 25, 2011    11:36 AM

Posted new topic in General forum   forum -statistical analysis of biological data

Hi fellow scientists I am inviting your opinions in starting a forum - statistical analysis of biological data.I strongly favour a forum like this with the increasingly complex analysis of data generated by HTS techniques like micro array and deep sequencing. Thanks. Biju Joseph

May 25, 2011    09:47 AM

Replied to topic in Cloning (Recombinant Gene Expression) forum   Ligation problem with XhoI site

Hi quixo I had problem with XhoI cloning in the past.I consider the enzyme to be not very friendly one. Could you try an altrenate site/enzyme?I might be wrong as well in the conclusion. Biju joseph

May 25, 2011    09:44 AM

Replied to topic in RNA forum   RNA Levels

Hi Annie The best way to quantitate RNA or DNA is always spectorphotometry - nanodrop or other methods.As far as reagent is concerned, it is difficult to predict which works best.You should find the best way in your lab/your hands by trying different reagents. Biju Joseph

May 20, 2011    02:09 PM

Posted new topic in Cell Signaling/Transduction forum   TGF beta ligands

Hi Could anybody let me know the best TGF beta ligands available for purchase?I have plan of ordering the TGF beta ligands for an assay. Thanks. Biju

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