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Pippuri
Member since: Jul 12, 2009
From: Georgia, United States
Status: Protein Chemistry & Glycobiology Moderator
My points: 1081    what's this
Name: [Privacy]
 


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Mar 9, 2012    11:18 AM

Replied to topic in Virology forum   help me for selection of best lentiviriseconcentration kit

Hi masomeh_es.haghi, Somebody else in my lab uses it. I don't usually concentrate my lentiviral particles. I found that using "spinoculation" (forcing the pseudo viral particles attached to the cell surface hence enforcing their entry via centrifugation) actually works decently in m ...

Mar 9, 2012    10:43 AM

Replied to topic in Virology forum   help me for selection of best lentiviriseconcentration kit

Hi masoumeh_es.haghi, Here is one without the ultracentrifugation step: Lenti-X concentrator (Clontech) Good luck.

Feb 26, 2012    06:04 AM

Replied to topic in Analytical Chemistry forum   mass spectrometry

Hi ghazaleh842000, There is no reason for one to see singals in MALDI but not in LC-MS/MS. If the peptides are there, they should be able to be detected regardless. Cheers.

Feb 15, 2012    01:38 PM

Replied to topic in Analytical Chemistry forum   mass spectrometry

Hi ghazaleh842000, If the mass spectrometer on which one acquires data has the resolution power, yer, on can tell the charge state of a specific peak- by looking at the distribution of the isotopic variants of that peak (But you really have to zoom-in in order to see that, hence on the first gimp ...

Feb 15, 2012    11:56 AM

Replied to topic in Analytical Chemistry forum   mass spectrometry

ghazaleh842000, I am not sure I understand fully your question. At the full ms level, there is no special "look" of each species. But if your full ms has multiple species on one spectrum, you should be able to back calculate for all of them share the same value of M, but vary in n value ...

Feb 15, 2012    07:07 AM

Replied to topic in Analytical Chemistry forum   mass spectrometry

Hi ghazaleh842000, For single charge species, the m/z value = [M+H], M being the mass of your target (says peptide, but can be anything), and H being mass of the hydrogen atom (1.0078). For doubly charge species, the m/z value = [M+2H]/2, M being the mass of your target (says peptide, but can ...

Feb 13, 2012    08:23 AM

Replied to topic in Protein Chemistry forum   mass spectrometry on Factor XIII

Hi ghazaleh842000, You need to collect MS/MS spectra (which contain information of b/y ion series specific for your peptides) for such assignment. I am not very familiar with MALDI system- for what I understand, since your sample is mixed with matrix prior to TOF-MS/MS analysis, there is no L ...

Feb 12, 2012    08:57 AM

Replied to topic in Protein Chemistry forum   mass spectrometry on Factor XIII

Hi ghazaleh842000, How long did you perform pepsin digestion? Have you tried to reduce the digestion time for B2?

Feb 12, 2012    08:57 AM

Replied to topic in Protein Chemistry forum   mass spectrometry on Factor XIII

Hi ghazaleh842000, How long did you perform pepsin digestion? Have you tried to reduce the digestion time for B2?

Feb 9, 2012    06:51 AM

Replied to topic in Protein Chemistry forum   mass spectrometry on Factor XIII

Hi ghazaleh842000, Can you pleasebe more specific about your approach? For instance, are you using MALDI or ESI based method? Are the sample analyzed on the whole protein level or have it been digested? How does the calibration/tuning conditions on the instrument? Cheers.

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