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Invitrogen Dynal Tech Support
Member since: Sep 01, 2009
From: Norway
Status: Frog Egg
My points: 31    what's this
Name: [Privacy]
 


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May 3, 2010    07:17 AM

Replied to topic in Pharmacology and Drug Discovery forum   Who have ever used the streptavidin coated magnetic beads from invitrogen

Hi There I am Kristina with Technical Support for Invitrogen Dynal and I'm happy to help with any Dynabeads related questions. I'm sorry to see that you are having problems with the streptavidin beads, and I hope we can figure out what the problem might be and get the binging working. Firs ...

Sep 22, 2009    06:34 AM

Replied to topic in Protein Detection (Western blots, gels, IP) forum   Immunoprecipitation Advice Needed

Hi The need for preclearing is dependent on the solid support (magnetic beads or sepharose/agarose) you are using for your IP. Some supports are more likely to bind non-specificly to certain proteins. I can only speak for Dynabeads and we do not recommend pre-clearing as part of a standard IP pro ...

Sep 3, 2009    06:53 AM

Replied to topic in Immunology forum   Regulatory T cell isolation

Hi Nargess Thank you for explaining in more detail what you are looking for. In case anyone is confused about Treg characterisation, here is a short summary: Treg cells are CD4+CD25+ and FOXP3+. However, activated T cells also have a transient expression of CD25 (caracterized as CD25low or ...

Sep 1, 2009    07:05 AM

Replied to topic in RNA forum   How much RNA is there in a cell?

Hello mRNA accounts for less than 4% of the total RNA in mammalian cells. Therefore, isolating only the mRNA can really improve results. You might not know the 'best kept secret in RNA isolation'? - Dynabeads oligo d(T). These beads capture super-pure poly(A) m-RNA from crude lystae or RNA preps. ...

Sep 1, 2009    06:56 AM

Replied to topic in RNA forum   How to isolate RNA-Protein complexes

Hi there Check out our web-page on isolation of RNA/DNA binding proteins using Dynabeads Streptavidin. There are several good references on the page: http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Nucleic-Acid-Purification-and-Analysis/napamisc/Capture-of-Biotinyl ...

Sep 1, 2009    06:45 AM

Replied to topic in Immunology forum   B lymphocyte Isolation from blood

Hi The best method of isolation will be determinded by your intended down-stream application. How pure do you need the cells to be? Should cells be 'untouched' Are you doing molecular or cellular studies? We have two great kits for B cell isolatin from human samples: Option 1: Dynabeads CD ...

Sep 1, 2009    06:22 AM

Replied to topic in Immunology forum   Biomagnetic separation of macrophages

Hi there I am a support scientist at Invitrogen Dynal. We have many different Dynabeads and a few different methods of detachment of the beads from the cells. In general, unless you are using a bead to which the antibody is attached via a molecule which can be competed out or digested, you will n ...

Sep 1, 2009    06:09 AM

Replied to topic in Immunology forum   PBMC Isolation

In my experience the key factor is to do the layering very carefully. Use a 50 ml pipette, tilt the tube so that you get a large surface of Ficoll to work on and let the diluted blood drip as slowly as possible down to the Ficoll. Once you have a centimetere or so of blood you can start to increase ...

Sep 1, 2009    06:00 AM

Replied to topic in Immunology forum   Regulatory T cell isolation

Hi We have great Treg isolation kits for both human and mouse samples. The isolation process consists of two steps: 1. Negative isolation of CD4+ cells from PBMC 2. Positive isolation of CD4+CD25+ cells from the CD4+ population. These cells are then detached from the beads, giving you both the ...