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patchclamper
Member since: Jan 15, 2010
From: United States
Status: Frog Egg
My points: 34    what's this
Name: [Privacy]
 


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Jun 21, 2010    12:40 PM

Posted new topic in Anatomy and Physiology forum   Monitoring the series resistance

Hi guys, For whole cell voltage clamp experiments, monitoring the series resistance is important for getting an idea on how well the voltage clamp is. I monitor the Rs for the whole experiment and discard the recordings in which the Rs increased more than 20%. Here I have several questions ...

Jun 7, 2010    05:12 PM

Replied to topic in Anatomy and Physiology forum   IPSC- frequency and amplitude

I am not an expert on this, try to explain: Increasing/decreasing in frequence means whatever happened altered the release probability of the presynaptic terminal through mechansims that change the Ca2+ concentration or vesicle release machinary. (increasing extracellular Ca2+ level, drug targeti ...

May 31, 2010    12:22 PM

Replied to topic in Anatomy and Physiology forum   Lose the seal when holding the membrane at -60mV

Still have the same problem, now I changed my amplifier to 700B, and everything works fine... But still have no idea why my old 200B will cause this "hyperpolarization induced seal lose"....Probably something wrong with the gain...

May 28, 2010    06:52 PM

Replied to topic in Anatomy and Physiology forum   Salt Crystals Blocking Recording Pipette--any ideas?

Blood cells are really easy to get into the tip too, so I immediately apply positive pressure as soon as the electrode is in the solution. Air bubble is another thing often trapped in the tip, I just suck it out, but this will cause some external solution to come in, how do you guys deal with tha ...

May 25, 2010    11:43 AM

Replied to topic in Anatomy and Physiology forum   what is the mobility of the methanesulfonate ion (MeSO4)?

According to the ion mobility table here (http://web.med.unsw.edu.au/phbsoft/mobility_listings.htm), it's 0.664

May 6, 2010    04:49 PM

Replied to topic in Anatomy and Physiology forum   Lose the seal when holding the membrane at -60mV

Hi Frere, I am still having this problem now, similarly, when I hyperpolarized the seal about -40mV, the input resistance decreased from 2 GOhm to about 15 MOhm. Whether the decreasing of input R is due to losing the attachment of membrane to the glass or due to going to whole cell directly is ...

Apr 19, 2010    09:13 AM

Replied to topic in Anatomy and Physiology forum   acute hippocampal slices

Hi, Just wondering have you solved the problem yet? I am having a similar problem..Thanks for replying

Apr 17, 2010    04:07 PM

Replied to topic in Anatomy and Physiology forum   Lose the seal when holding the membrane at -60mV

No, The seal is very stable, until I hyperpolarize the cell... Yesterday I found that my fast electrode capacitance compensation is off, it seems when I hyperpolarize the cell the compensation circuits is generating some osllilation which will potentially damage the seal. Today I will do a record ...

Apr 15, 2010    03:18 PM

Posted new topic in Anatomy and Physiology forum   Lose the seal when holding the membrane at -60mV

Hi everyone, After getting a seal, I hold the membrane at -60mV then break in. There was no problem with it before, however recently, when I hold the membrane at negative potential, I lost the seal almost everytime (some times go to whole cell directly). Do you guys have any idea what are the pot ...

Apr 10, 2010    09:39 PM

Posted new topic in Anatomy and Physiology forum   Share some patch clamp protocols I found

Patch-clamp recording from mossy fiber terminals in hippocampal slices http://www.nature.com/nprot/journal/v1/n4/full/nprot.2006.312.html Dendritic patch-clamp recording http://www.nature.com/nprot/journal/v1/n3/full/nprot.2006.164.html Paired-recordings from synaptically coupled cortica ...

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