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Shereen Cynthia
Member since: Dec 09, 2008
From: Puducherry, India
Status: Frog Egg
My points: 18    what's this
Name: [Privacy]
 


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Feb 25, 2010    08:43 AM

Replied to topic in Protein Detection (Western blots, gels, IP) forum   Immunofluorescence

40 X.. I dont think the problem is with the microscope. I have done immunoflurescence a year back and could take good and clear photographs. I observed those slides today and they are as good as they were. It is going wrong soemwhere else. Could it be the moutant? I have prepared it new and the ...

Feb 25, 2010    08:13 AM

Posted new topic in Protein Detection (Western blots, gels, IP) forum   Immunofluorescence

My Immunofluorescence slides have a cloudy appearance. Can anyone explain me the reason? The slides do fluoresce, but have a cloudy appearance and the cells (Testis section) are not clearly visible. I use the standard immmunofluorescent procedure and mount using glycerol jelly. When I prepare H ...

Dec 10, 2008    01:27 AM

Posted new topic in Biochemistry forum   Dissolution of bisphenol A

Could anybody tell the appropriate vehicle for dissolving bisphenol A?