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ldemers' Bench [how it works]
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ldemers
Member since: Jan 12, 2006
From: United States
Status: Frog Egg
My points: 0    what's this
Name: ldemers
 


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Jan 13, 2006    11:41 AM

Replied to topic in Antibody Based Technologies forum   Assay Development

The protein I have in mind is a 9.5kD ribosomal subunit zinc-finger protein that is passively secreted from cells. The primary antibody is from rabbits. We are having problems with the antibody sticking to microtiter plates in order to develop a sandwich type ELISA. Any suggestions about plate coa ...

Jan 12, 2006    06:39 PM

Posted new topic in Antibody Based Technologies forum   Assay Development

We have access to a purified tumor associated peptide along with polyclonal antibody to develop an ELISA. We would like a detailed protocol to develop an ELISA quickly using a microtiter plate format. Would like specific recommendations regarding how to coat the plates with antibody to insure that ...

Jan 12, 2006    06:39 PM

Posted new topic in Antibody Based Technologies forum   Assay Development

We have access to a purified tumor associated peptide along with polyclonal antibody to develop an ELISA. We would like a detailed protocol to develop an ELISA quickly using a microtiter plate format. Would like specific recommendations regarding how to coat the plates with antibody to insure that ...