Maybe there should be a distinction made between sales and marketing functions. Marketing is (supposed to be) about understanding what your customers want, tailoring your offering in such a way that is satisfys this demand and ideally "delights " them. This positive experience would then ...

Assuming that your DNA sequencing confirmed that your deletion had been made, the reading frame had not been changed and that the polyadenylation signals are still there, I would look at the antibody you are using. ie. If it is a monoclonal, is the epitope it detects still in your 10Kd and 15Kd ...

Hi Cambsci, Sorry for the delay in answering - email problems! I was wondering whether you had ever looked at the purity of the virus concentrated using Lenti-X. Most people will look at the infectious titre before and after concentration and be happy (or not) with that. However, the main se ...

The Levey-Jennings chart enables a graphic readout of the experimental variance present in an assay. It allows you to analyse the blanks and standards and if these are OK, tells you that the results of the assay can be interpreted. The link below seems to have LJ chart templates that can be downlo ...

Hi Scolix, It would be great if you could you post the ultracentrifugation conditions and solution concentrations you use for this lenti concentration? Thanks. I lent my Lenti X Concentrator to a colleague today who will be trying it out. Perhaps it would be good if she compared it to your ...

I used to work on retroviruses and did quite a lot of retroviral vector cloning and based on this experience (some of it quite painful) I would start off with the following points: 1. You did the control transfection using an expression vector. It did not work (despite a similar GFP expression ...

Do I understand correctly that the virus you attempted to rescue was based on the expression construct that you transfected into cells (which did not result in gp160 expression)? Was the expression vector that did not work in the transient transfections of the same type as the control (YU-2 En ...

If, for some reason I am not able to proceed with the antibody steps of the western blot, I would do a >30minute incubation in the PBS/skimmed milk solution (blocking buffer), Place the blot between 2 pieces of thick Whatman paper, put the "sandwich" ina Zip-lock plastic bag and store ...

The answer to the first question is, viral genome copies. Depending on the type of virus you are analysing, there will be varying proportions of empty capsids and partially filled ones. You can only detect what is there and so this will be the full and partially full virions. Although I've never ...

If you are looking for non-clinical studies, PubMed lists very many. Since the disease caused by the virus is self-limiting and really only a danger to pregnant women, there may be rather less work done on it than on Hep C for example. It is also likely that since the virus spreads as a result o ...