My suggestion would be to contact the sales rep in your area for Nikon scopes. Generally, sales people have lots of experience in answering these kind of questions. We have a different scope, but nonetheless, our choices for what cameras could be connected was limited by what couplers were ava ...

I think if you break up the agarose enough that cell will find their own way out into the medium. I'm not aware of any way to melt, even low-melting point agarose, and get viable cells out. I always use methylcellulose instead of agarose when I want to harvest colonies.

I'm getting the impression your experiment may actually be working very well. I would trust the negative controls and set them within the first decade and count everything else as positive. Different cell types are likely to express different levels of eGFP and even the same cells will have a dist ...

First of all, it is important to remember that flow data is not the same from machine to machine or even on the same machine with different settings. You can increase or decrease your voltages as much as you like to increase or decrease negative controls. Your aim should be to use as much of the a ...

It is possible to have widely different cells in terms of size and complexity in a single tissue. Often in a cell preparation you may have red cells and leukocytes from blood that are likely to be the smaller cells and then you have the various tissue specific cells. I would first try to really ...

Your mother would have to be genotype A/O +/-, phenotype A+ Your father would have to be at least heterozygous for O and Rh-, e.g. B/O +/- (phenotype B+) but capable of yielding offspring that are O- and O+.

Aabel for charting numerical data. FlowJo for flow cytometry data analysis and graphing. iVision for microscopy data, which are stored and cataloged in iPhoto. Individual charts and pictures are put together in OmniGraffle with an occasional assist by Graphics Converter.

Thanks for letting us know what is working for you. It is nice to hear feedback so we all can learn something.

1) As I tried to suggest in my last post, most of the time in my experience with hematopoietic progenitors it is desirable to have the cells concentrated, although I've never had them settle out completely. I've never worked with such small wells, it is possible that the static charges and meniscus ...

What volumes and plate sizes are you using? Generally it is desirable to have the cells in a narrow plane to make counting easier (by avoiding the need to continuously refocus on different focal planes). I place cells in 0.5ml upper layer over a 1ml underlayer in 35mm2 plates and, for 60mm2 plates ...