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Carson O Genic
Member since: Jun 22, 2005
From: California, United States
Status: Stem Cell Biology Moderator
My points: 438    what's this
Name: Carson O Genic
 


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May 1, 2013    04:06 PM

Replied to topic in Stem Cell Biology forum   Identification of human hepatic progenitor cells

Jason, For endodermal differentiation one can use FoxA2 and SOX17. Albumin and alph-fetoprotein are two of the main hepatocyte products used to ID hepatocytes and their fetal equivalents, respectively. Off course there are many other genes such as transferrin, hepatocyte nuclear factos (e.g. ...

Mar 8, 2013    10:49 AM

Replied to topic in Stem Cell Biology forum   Isolation of Cardiac stem cells in Murine amnimals

Sorry, I don't know anything specific about this subject, so I'm just replying to bump the topic up list again.

Oct 14, 2012    06:10 PM

Replied to topic in Stem Cell Biology forum   Mesenchymal stromal cells from amnion - differentiation

If you feel your going to loose them, then I would passage.

Aug 13, 2012    02:21 AM

Replied to topic in Immunology forum   Cytokine concentrations helpppppp

Your Explantation of The math is a Bit Off. Nonetheless, 20ul added to 180ul is a 1/10 dilution and if you do this three Times in a Row then you have it.

Aug 12, 2012    12:35 AM

Replied to topic in Immunology forum   Cytokine concentrations helpppppp

Yes your 1/10 dilution will yield 100ng/ml, but remember if you remove 20ul for the next dilution that you will end up with 180ul final volume. if you dilute it back to 200ul then your concentration will be incorrect.

May 8, 2012    11:53 AM

Replied to topic in Microscopy and Imaging forum   Double Immunofluorescence

The second-step antibodies should be no probolem. My main concern is that the polyclonal may have some cross-reactivity with the other-sepcies antigen if they are similar enough.

Apr 19, 2012    04:55 PM

Replied to topic in Cell Culture and Tissue Culture forum   rat mesenchymal stem cells

Your question is difficult to answer because there are so many variables and what mRNA your analyzing is not specified. However, rule of thumb is that the longer you culture your cells the less 'normal' they will become. Indeed, there are likely significant transciptional changes in your cells in ...

Apr 19, 2012    04:49 PM

Replied to topic in Cell Culture and Tissue Culture forum   An alternative to magnetic bead separation for monocytes/macrophages

You may try enrichment by Ficoll and adherence on plastic for positive selection. Depends on what the other tissues are and what kind of other light-density cells may adhere to plastic.

Mar 14, 2012    11:52 AM

Replied to topic in Stem Cell Biology forum   ES cell transfection / culture

Moved to stem cells forum, with a link to the old post. They sound a little bit like embroid bodies, i.e. differentiating ES cell clones.

Mar 9, 2012    11:52 AM

Replied to topic in RNA forum   !00 classic review articles for stem cells

Thanks for the post. I moved it under stem cells, as that seems to be a better fit.

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