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varsha
Member since: Dec 10, 2006
From: North Carolina, United States
Status: Microbiology & Proteomics Moderator
My points: 412    what's this
Name: [Privacy]
 


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Nov 23, 2008    05:47 PM

Replied to topic in Microbiology forum   Microbiology

[quote=][/quote] Try the protocols in these papers, Good luck! http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=92588 https://commerce.metapress.com/content/v32442737w270700/resource-secured/?target=fulltext.pdf&sid=dap1j245rieayoah1gly15mv&sh=www.springerlink.com http://aem.as ...

Nov 22, 2008    06:07 PM

Replied to topic in Microbiology forum   Membrane Filtration and Bacterial Counts

[quote=][/quote] Try these sites: http://www.pall.com/laboratory_20841.asp http://filebox.vt.edu/users/chagedor/biol_4684/mfcoli.html

Nov 21, 2008    04:04 PM

[quote=][/quote] GM crops may have both positive and negative impact on the environment. Many of the varieties are targeted either for resistance to disease or pests and may have high nutritional requirements which would be more carbon positive than traditional varieties. GM crops like BT (Ba ...

Nov 21, 2008    03:37 PM

Posted new topic in Model Organisms forum   Nobel Prize in Chemistry goes to a C. elegans Scientist

Dr Martin Chalfie at Columbia University won the Nobel Prize in Chemistry along with Osamu Shimomura and Roger Y. Tsien in Oct 2008. http://nobelprize.org/nobel_prizes/chemistry/laureates/2008/index.html Dr Chalfie is credited with the pioneering the use of GFP in C. elegans. Now almost ever ...

Nov 21, 2008    03:26 PM

Replied to topic in Protein Detection (Western blots, gels, IP) forum   cytosol protein

[quote=][/quote] Hi Lila. I use antibody to GAPDH (from Cell Signalling http://www.cellsignal.com/products/2118.html), a cytosolic enzyme, to check the purity of cytosolic fraction. You may also use markers for nuclei (lamin B, http://www.abcam.com/) ER (grp78, www.AssayDesigns.com), mitochondri ...

Nov 21, 2008    03:03 PM

Replied to topic in Protein Detection (Western blots, gels, IP) forum   Western Blotting Help

I have done Westerns for 16 kDa protein (Hsp16) from C. elegans. I do a 1h wet transfer (Bio-Rad setup) at 100v/250mA in cold room onto NC membranes. Blocking with 3% milk in TBS-T primary for 1h at RT (depends upon the affinity of ab) secondary (anti-rabbit-HRP from SIGMA at 1:10,000 dilution) ...

Nov 21, 2008    10:35 AM

Replied to topic in Proteomics forum   Protein-protein interaction

that is great. please provide URL or at least a paper reference in your posts. for the above post it would go like this. BOND http://bond.unleashedinformatics.com/ APID http://bioinfow.dep.usal.es/apid/index.htm DIP http://dip.doe-mbi.ucla.edu/ String http://string.embl.de/ http:/ ...

Nov 21, 2008    08:57 AM

Posted comment on blog   Jurassic Park, For Real???

454 is a large-scale sequencing system most frequently used for whole genomes. A small fragment of DNA (e.g fragments chopped genome from a newely isolated bacterium) is blunted and ligated to adapters on both side, one of the adapters is biotinylated and can be captured onto a streptavidin bead. No ...

Nov 20, 2008    01:48 PM

Replied to topic in General forum   Kindly help for downloading the following pdf

[quote=][/quote] They are not available to me either. You could write to the corresponding author to send you the PDFs. Good luck!

Nov 20, 2008    09:05 AM

Replied to topic in Proteomics forum   Protein-protein interaction

Could you put a link to each of the databases and tell us the specific features or output which makes them better.

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