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TheFFM
Member since: Dec 06, 2008
From: Ohio, United States
Status: Administrator & Neuroscience, Biophysics, and Microscopy Moderator
My points: 1353    what's this
Name: Fraser Moss
 


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Mar 25, 2010    12:07 AM

Replied to topic in Anatomy and Physiology forum   ...want to understand what leak current mean and why to compensate

Leak currents are those that passively flow across the cell membrane because the channels that allow their passage are always open. The leak channels do not gate the current like voltage or ligand activated channels, but allow the flow of ions all the time. There are however different types of ...

Mar 24, 2010    11:29 PM

Posted new topic in The Lighter Side of Science forum   Mitosis Raps

There plenty of them out there Keep sharing them here

Mar 24, 2010    11:29 PM

Posted new topic in The Lighter Side of Science forum   Mitosis Raps

There plenty of them out there Keep sharing them here

Mar 24, 2010    11:20 PM

Posted new topic in The Lighter Side of Science forum   DNA transcription in the medium of dance
Mar 24, 2010    11:20 PM

Posted new topic in The Lighter Side of Science forum   DNA transcription in the medium of dance
Mar 24, 2010    11:18 PM

Posted new topic in The Lighter Side of Science forum   Songs inspired by DNA - you'd be surprised how many there are

Here are some DNA inspired songs - I'm sure there are plenty more. Keep posting yours after this post And why is this dude in the lab with his shirt off?

Mar 24, 2010    11:18 AM

The purpose of an agar salt bridge is to provide an electrical connection to the bath solution while minimizing the transfer of ions or solute from the electrical environment. Use the following procedure to create salt bridges. This procedure involves: 1) formation of bridges 2) preparing ...

Mar 19, 2010    02:22 PM

Replied to topic in Cloning (Recombinant Gene Expression) forum   Expression of my fusion protein!

I think your boss is probably right. It looks like somewhere in your linker you have probably introduced a cleavage site and the enzyme that should be fused is being cleaved and degraded OR not detected because you are probing with Anti HIS and the cleaved enzyme has no HIS epitope. When you ...

Mar 19, 2010    01:43 PM

Replied to topic in Cell Culture and Tissue Culture forum   contamination?

Probably mycoplasma, but if you could upload an image of the cultures that might help identification. See this discussion from earlier today on removing Mycoplasma http://www.scientistsolutions.com/t14180-mycoplasma+in+cell+culture.html To detect and confirm for Mycoplasma contamination th ...

Mar 19, 2010    11:03 AM

Replied to topic in Cell Culture and Tissue Culture forum   Mycoplasma in cell culture

Here are some Cell Freezing Protocols that you can find on this site http://www.scientistsolutions.com/a6978-protocol-derivation+of+human+skin+fibroblast+lines+for+feeder+cells+of+human+embryonic+stem+cells.aspx http://www.scientistsolutions.com/a7243-protocol-cell+freezing+without+pre_coolin ...

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