A method for performing microchip-based PCR with an external heat source such as a TEC or resistive heater has been described. Although flow-through PCR and other novel methods of microchip-based thermocycling have been described, external heating of the microchip is still one of the simplest and most reliable methods of performing PCR in lab-on-a-chip systems. Additionally, external heating of the microchip allows for seamless integration of the PCR amplification component with existing microchip designs. Fabrication steps and amplification methods have been successfully used for chip-based PCR amplification (Herold and Rasooly, 2009. Lab-on-a-Chip Technology. ISBN: 978-1-904455-47-9).
See also: Miniaturized PCR-based biosensors
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