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Membrane protein quantification

hermione


Posted 7/19/2012 2:03:24 PM 		Hi friends,

I need help with membrane protein quantification. I already use a  Kit for protein quantification, but membrane proteins are insoluble. So, I am not sure how to quanitify it using the same kit.


I wanna know whether boiling the protein in NaOH might help?



 
Pippuri


Posted 7/22/2012 7:57:39 AM 		Hi hermione,



Two questions:

1) What kind of buffer is your membrane proteins stored in?

2) What kind of kit you are using right now?

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