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How long can the lysiates in sample buffer be useable withperfect detection

farzanehrostami


Posted 5/8/2012 12:56:05 AM 		My supperviser is attending to bring some lysate samples from another country in sample buffer . He is ging to use these sample for several western blotting. Is it true?
ARGERINE


Posted 5/8/2012 4:54:32 AM 		 hi 



Alternatives are:



1) It's frozen in lysis buffer

2) It's maintained at low temperature (subzero)

3) It's in lyophiliized form that he can resuspend 

4.) Sample buffer in boiled form to inactivate the proteases





Hope that helps
farzanehrostami


Posted 5/8/2012 11:40:10 PM 		Hi  ARGERINE.

Thanks for your kind reply.

But I still have another question.  He is going to perform several western blotting in different days.Do you agree with several freezing and defreezing processes? Are you sure, this method can protect proteins ?
ARGERINE


Posted 5/9/2012 10:30:13 AM 		 Hi 



Probably yr supervisor has multiple aliquotes of  it. If he hasnt then when u first thaw the sample maka aliquots. 
farzanehrostami


Posted 5/12/2012 5:00:43 AM 		Thanks a lot

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