RNA isolation from bone

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manish
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RNA isolation from bone

Does any body have a protocol for how to isolate RNA from bone tissue (any bone)? Appreciate any help with this regard!
Manish

vanishing
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I found this protocol that

I found this protocol that uses trizol:
http://www.stanford.edu/group/sjeffreylab/PDF%20Files/RNA_isolation.pdf

Or try this paper:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12401828&dopt=Abstract

And this link on protocol online (But that is me cheating, someone has answered the question for me):
http://www.protocol-online.org/biology-forums/posts/1141.html

Hope that helps!

manish
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Thanks a lot! Manish

Thanks a lot!
Manish

Tony Rook
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manish:

manish:

Please refer to this previous posting on Scientist Solutions.

RNA Isolation from Bone Protocols

docqian
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I have used RNeasy lipid

I have used RNeasy lipid Tissue Mini Kit from QIAGEN to isolate RNA from bone. It works well.

Ayala67
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docqian wrote:I have used

docqian wrote:

I have used RNeasy lipid Tissue Mini Kit from QIAGEN to isolate RNA from bone. It works well.

HI! I want to isolate RNA from turtle shell bone. may be you have some tips for me? Thanks!