DGGE problem

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coraline de sousa
coraline de sousa's picture
DGGE problem

 I have some problems for running samples into the gel... I explain: My company produces and commercializes organic intrants which are based on a complex of microorganisms (thousand of microorganisms). Once into the soil these microorganisms let the production of humic acids and help the plant to develop well. I extracted DNA (EZNA DNA soil, Omega) from fraction of soil treated or not with our organic soil, I performed PCR (16S RNA amplification), I loadded into the gel (45-65 % gradient)but the samples did not run into the gel even there are bubles... So I tried different things: I remove glycerol used to make the gel, I change the acrylamide stock, I change the detergent solution...Do you have any idea?Thanks Pauline