What are the main procedures of iTRAQ/TMT experiment, and which instruments are used?

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Marry Leon
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What are the main procedures of iTRAQ/TMT experiment, and which instruments are used?

The experimental procedures include protein extraction, concentration determination and SDS-PAGE gel detection, quality inspection report, proteolysis, isotope labeling, one-dimensional chromatographic separation, lyophilization freeze-drying, high-resolution LC-MS/MS mass spectrometry, search qualitative and quantitative analysis, differential protein screening, differential protein bioinformatics analysis and complete report collation.