What is the ideal speed for harvesting 400 mL of bacterial culture from induced pellet ( 1mM iptg) ?
Some protocols say 4000 rpm at 20 minutes or
others at 14000 rpm at 20 minutes ?
I obtained my pellet centriguginf at 14.000 for 12 mins... would that be ok ?
is there any difference from a 14.000 rpm pellet from a 4.000 rpm pellet ?