Dissolving curcumin

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misha
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Dissolving curcumin

I intend to do some experiments with curcumin. If someone know the best way to dissolve this chemical for rat diet as well as for tissue culture application.

macbride
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We dissolve our curcumin

We dissolve our curcumin analogs in molecular biology grade DMSO for cell culture applications.

vanishing
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but be careful with DMSO in

but be careful with DMSO in tissue culture
do not exceed a concentration of 1-2 (3=absolute maximum!!!) per mille

jachmoody
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1-2 what unit were you

1-2 what unit were you refering to?

jim

Fraser Moss
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I try not to use more than 0

I try not to use more than 0.1% DMSO conc in the final solution. I believe it is safe to go up to 0.5% if necessary. Just do a negative control with DMSO and no drug to confirm this. Of course the more concentrated you make your stock solution the easier it is to achieve a low final DMSO concentration.

Fraser Moss
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Here are some methods for

Here are some methods for your rodents

1) Curcumin is dissolved in Me2SO, and then ethanol is added and mixed with PBS (for 1 mg per 100 l of curcumin solution containing 5.4 lofMe2SO, 44.6 l of ethanol, and 50 l of PBS, pH 7.2). During brief anesthesia with halothane, a 25-gauge feeding needle (or PE-90 tubing) is used to infuse 45 mg/kg curcumin into the mouse stomach daily for 3 days, with the final dose delivered 2 h before the measurement of nasal PDs.

2) Curcumin powder is mixed with Alimentum (50 mg/ml) and sonicated for 30 s just before oral delivery by presenting the liquid at the tip of an Eppendorf pipette to non-anesthetized mice. The presented liquid is rapidly ingested by most mice. Curcumin is given at 0, 45, or 100 mg/kg three times a day for 3 days, with the final dose delivered 2 h before the measurement of nasal PDs.

3) The third method is the same as the second one, except that Peptamen is used instead of Alimentum as the delivery vehicle.

See - J Biol Chem. 2004 Sep 24;279(39):40629-33
and Egan, M. E., et al (2004) Science 304, 600602

Are you intersted in the CFTR rescue or do you have another intersting application for the spice?

macbride
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You can go up to 1% DMSO in

You can go up to 1% DMSO in cell culture, although I have noticed certain cells lines are less tolerant of this than others.

saswati1
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For the sigma product (C-1386

For the sigma product (C-1386), both alcohol and DMSO work for solubility

misha wrote:

I intend to do some experiments with curcumin. If someone know the best way to dissolve this chemical for rat diet as well as for tissue culture application.
mcdeena2007
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Hi, I need to dissolve

Hi, I need to dissolve curcumin in 0.5% DMSO. Does that mean dissolve 100mg of curcumin in 0.5mL of DMSO?