Superdex 200

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pauline teoh
pauline teoh's picture
Superdex 200

I am doing about the nanorods conjugate antibody research work. I tried to purify my end product by using self-packed superdex 200 colum. I found that the nanorods solution stuck at top of the gel, and no have antibody eluted out. I cofirmed the fraction by using SDS-PAGE method.
 Any suggestion about that. I would apreaciate the suggestion.
Thanks.