Incubation time and Drug treatment``

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vdas
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Incubation time and Drug treatment``

Hi,
Could anyone kindly tell me what concentration should I use to treat cells with drugs for WB analysis. I have my MTT IC50 values and flow cytometry numbers also. But I was wondering at what concentration (2, 3..times the IC50 concentration) should I treat mt cells with and for how long (12, 24..hrs incubation).
Is it better to treat cells with drug in a 24 well plate or a petri dish. Could any pls tell what can be the approx measurements !!
Thanks,