I want to know that why caliberation curve is made in different sovent and buffers systems during preformulation studies.
calibration curve is made for defining the knopwn range of conc of solute in a solution . if solution is composed of more than one variant it is better to rule out that.
Calibartion curve ismade i differant solvent to nullify if any interferance of the solvent.
The calibration curve shows a linear relation with the type of analysis you are performing as well as it will tell you the range of your test...in case of Protein formulation calibration curve shows a linear relation between concentration and absorbance. It shows that Beer Lambert law is obeyed in the concentration range in case of assay reproducible is checked with inter-day and intra-day variation of the slopes of the calibration curves