Hallo out there!
Could somebody please help me to understand how to calculate the osmolarity of a buffer?
More precise for the following buffer :
Thank you in advance,
The osmolarity of a solution may be given as the sum of (yi)(ni)(Ci), where yi is the osmotic coefficient of solute i, n is the number of particles that solute i dissociates into and Ci is the molarity of solute i.
The osmotic coefficient (yi) has a range of 0 to 1 - the osmotic coefficient will be 1 when the solute is completely dissolved and 0 when insoluble.
so the osmolarity of your solution should be
2 x 120 (NaCl) + 3 x 0.5 (CaCl2) + 2 x 2.5 (KCl) + 3 x 1 (MgCl2) + 1 x 10 (Glucose) = 259.5 mOsm/L
Red text corrected typo on original post as stated below
A small correction, the osmolarity is 259.5 mOsm/L.
Indeed ... Good typo spot
I will correct the original reply