Any Liebermann-Burchard reaction expert here?

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amalina
amalina's picture
Any Liebermann-Burchard reaction expert here?

My standard failed to react but my sample doing well. There's no colour changes for my standard but my sample doing well as it turn to deep green. I think i've prob with my cholesterol standard. Is there any specific standard for this test? For this test I'm using SIGMA CHOLESTEROL, approx > 95% GC which we also using this cholesterol in feeding the rabbit to induce hyperchol condition.